ArticleProceedings of the National Academy of Sciences of the United States of America1994
The B1C8 protein is in the dense assemblies of the nuclear matrix and relocates to the spindle and pericentriolar filaments at mitosis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 61 citations in OpenAlex.
- Article
- Article
- The Ultrastructural Signature of Human Embryonic Stem Cells.Journal of cellular biochemistry · 2017Article
- Coordinated Dynamics of RNA Splicing Speckles in the Nucleus.Journal of cellular physiology · 2016Article
- Use of HCA in subproteome-immunization and screening of hybridoma supernatants to define distinct antibody binding patterns.Methods (San Diego, Calif.) · 2016Article
- Multifunctional RNA processing protein SRm160 induces apoptosis and regulates eye and genital development in Drosophila.Genetics · 2014Article
- Nucleoskeleton of early bovine embryos and differentiated somatic cells: an ultrastructural and immunocytochemical comparison.Histochemistry and cell biology · 2004Article
- In vitro FRAP reveals the ATP-dependent nuclear mobilization of the exon junction complex protein SRm160.The Journal of cell biology · 2004Article
- The dynamic association of RCC1 with chromatin is modulated by Ran-dependent nuclear transport.Molecular biology of the cell · 2004Article
- The spatial targeting and nuclear matrix binding domains of SRm160.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- SRm160 splicing coactivator promotes transcript 3'-end cleavage.Molecular and cellular biology · 2002Article
- The acute myeloid leukemia-associated protein, DEK, forms a splicing-dependent interaction with exon-product complexes.The Journal of cell biology · 2000Article
- The SRm160/300 splicing coactivator subunits.RNA (New York, N.Y.) · 2000Article
- The SRm160/300 splicing coactivator is required for exon-enhancer function.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Phosphoinositide signaling pathways in nuclei are associated with nuclear speckles containing pre-mRNA processing factors.Molecular biology of the cell · 1998Article
- A coactivator of pre-mRNA splicing.Genes & development · 1998Article
- A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Protein 4.1 is a component of the nuclear matrix of mammalian cells.The Biochemical journal · 1995Article
- The nuclear matrix phosphoprotein p255 associates with splicing complexes as part of the [U4/U6.U5] tri-snRNP particle.Nucleic acids research · 1995Article
- Association of nuclear matrix antigens with exon-containing splicing complexes.The Journal of cell biology · 1994Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The B1C8 monoclonal antibody detects a 180-kDa nuclear matrix-specific protein. The protein is a component of the dense, metabolically active bodies or assemblies revealed by resinless section electron microscopy of the nuclear matrix. These assemblies are scattered through the nuclear interior, enmeshed in a complex network of 11-nm filaments. Resinless section electron microscopy of immunogold-stained nuclear matrix preparations shows B1C8 located in many but apparently not all the assemblies. In this regard, the B1C8 antigen resembles previously studied nuclear matrix proteins such as the H1B2 protein. The speckled pattern of nuclear immunofluorescence by B1C8 reflects this labeling of the dense assemblies in the nuclear matrix. Somewhat unusual is the faint staining of cytoplasmic microtubules by B1C8, which appears to be due to a weakly cross-reacting protein. During cell division, the B1C8 antigen redistributed drastically, showing the dispersion of nuclear matrix assemblies at mitosis. Speckles of B1C8 fluorescence first coalesced at prophase within the nuclear interior and then scattered into numerous cytoplasmic speckles by prometaphase. At metaphase, the B1C8 speckled cytoplasmic staining had become even more widely distributed and finely grained. Also, intense labeling appeared at the mitotic pole and on the spindle fibers themselves. The reassembly of B1C8 antigens into larger cytoplasmic speckles began at anaphase and finally, at telophase, most B1C8 labeling redistributed into speckles in the re-forming nuclei.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.