ArticleNucleic acids research1993
Stimulation of gene expression by introns: conversion of an inhibitory intron to a stimulatory intron by alteration of the splice donor sequence.
Article in Nucleic acids research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
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- Effects of Genetic Variants of Nuclear Receptor Y on the Risk of Type 2 Diabetes Mellitus.Journal of diabetes research · 2019Article
- A highly significant association between Cathepsin S gene polymorphisms rs12068264 and chronic obstructive pulmonary disease susceptibility in Han Chinese population.Bioscience reports · 2018Article
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- Genetic variants of CDH13 determine the susceptibility to chronic obstructive pulmonary disease in a Chinese population.Acta pharmacologica Sinica · 2016Article
- Genetic variations in RORα are associated with chronic obstructive pulmonary disease.Journal of human genetics · 2014Article
- Association between fibroblast growth factor 7 and the risk of chronic obstructive pulmonary disease.Acta pharmacologica Sinica · 2012Article
- InTRONs in biotech.Molecular biotechnology · 2011Review
- Arginine-rich regions mediate the RNA binding and regulatory activities of the protein encoded by the Drosophila melanogaster suppressor of sable gene.Molecular and cellular biology · 2000Article
- Inhibition of the mammalian transcription factor LSF induces S-phase-dependent apoptosis by downregulating thymidylate synthase expression.The EMBO journal · 2000Article
- Packaging of intron-containing genes into retrovirus vectors by alphavirus vectors.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Regulated expression and RNA processing of transcripts from the Srp20 splicing factor gene during the cell cycle.Molecular and cellular biology · 1997Article
- Dual function of the intron of the rat insulin I gene in regulation of gene expression.Diabetologia · 1996Article
- Comparable processing of beta-lactoglobulin pre-mRNA in cell culture and transgenic mouse models.Molecular & general genetics : MGG · 1996Article
- The hepatitis B virus posttranscriptional regulatory element is composed of two subelements.Journal of virology · 1996Article
- Sequence of the polypyrimidine tract of the 3'-terminal 3' splicing signal can affect intron-dependent pre-mRNA processing in vivo.Nucleic acids research · 1996Article
- Splicing signals are required for S-phase regulation of the mouse thymidylate synthase gene.Molecular and cellular biology · 1996Article
- Isolation of a polyubiquitin promoter and its expression in transgenic potato plants.Plant physiology · 1995Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
Efficient expression of many mammalian genes depends on the presence of at least one intron. We previously showed that addition of almost any of the introns from the mouse thymidylate synthase (TS) gene to an intronless TS minigene led to a large increase in expression. However, addition of intron 4 led to a reduction in minigene expression. The goal of the present study was to determine why TS intron 4 was unable to stimulate expression. Insertion of intron 4 into an intron-dependent derivative of the ribosomal protein L32 gene did not lead to a significant increase in expression, suggesting that its inability to stimulate expression was due to sequences within the intron. Deleting most of the interior of intron 4, improving the putative branch point, removing purines from the pyrimidine stretch at the 3' end of the intron, or removing possible alternative splice acceptor or donor sites within the intron each had little effect on the level of expression. However, when the splice donor sequence of intron 4 was modified so that it was perfectly complementary to U1 snRNA, the modified intron 4 stimulated expression approximately 6-fold. When the splice donor site of TS intron 1 (a stimulatory intron) was changed to that of TS intron 4, the modified intron 1 was spliced very inefficiently and lost the ability to stimulate mRNA production. Our observations support the idea that introns can stimulate gene expression by a process that depends directly on the splicing reaction.
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