ArticleMolecular and cellular biology1994
Two different sequence elements within exon 4 are necessary for calcitonin-specific splicing of the human calcitonin/calcitonin gene-related peptide I pre-mRNA.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Adaptations to a subterranean environment and longevity revealed by the analysis of mole rat genomes.Cell reports · 2014Article
- Transposable elements in disease-associated cryptic exons.Human genetics · 2010Article
- Repression of prespliceosome complex formation at two distinct steps by Fox-1/Fox-2 proteins.Molecular and cellular biology · 2008Article
- Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing.Molecular and cellular biology · 2007Article
- Both U2 snRNA and U12 snRNA are required for accurate splicing of exon 5 of the rat calcitonin/CGRP gene.RNA (New York, N.Y.) · 2004Article
- Alternative splicing in the alpha-galactosidase A gene: increased exon inclusion results in the Fabry cardiac phenotype.American journal of human genetics · 2002Article
- The RNA binding protein YB-1 binds A/C-rich exon enhancers and stimulates splicing of the CD44 alternative exon v4.The EMBO journal · 2001Article
- Control of hnRNP A1 alternative splicing: an intron element represses use of the common 3' splice site.Molecular and cellular biology · 2000Article
- Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis.Microbiology and molecular biology reviews : MMBR · 1999Review
- Polypyrimidine tract-binding protein positively regulates inclusion of an alternative 3'-terminal exon.Molecular and cellular biology · 1999Article
- CA- and purine-rich elements form a novel bipartite exon enhancer which governs inclusion of the minute virus of mice NS2-specific exon in both singly and doubly spliced mRNAs.Molecular and cellular biology · 1999Article
- Regulation of alternative polyadenylation by U1 snRNPs and SRp20.Molecular and cellular biology · 1998Article
- Regulation of sex-specific selection of fruitless 5' splice sites by transformer and transformer-2.Molecular and cellular biology · 1998Article
- A short sequence within two purine-rich enhancers determines 5' splice site specificity.Molecular and cellular biology · 1998Article
- Splicing defects in the COL3A1 gene: marked preference for 5' (donor) spice-site mutations in patients with exon-skipping mutations and Ehlers-Danlos syndrome type IV.American journal of human genetics · 1997Article
- A splicing enhancer in the 3'-terminal c-H-ras exon influences mRNA abundance and transforming activity.Journal of virology · 1997Article
- The regulation of splice-site selection, and its role in human disease.American journal of human genetics · 1997Review
- Identification of a new class of exonic splicing enhancers by in vivo selection.Molecular and cellular biology · 1997Article
- An intron element modulating 5' splice site selection in the hnRNP A1 pre-mRNA interacts with hnRNP A1.Molecular and cellular biology · 1997Article
- Architectural limits on split genes.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The calcitonin (CT)/calcitonin gene-related peptide I (CGRP-I) gene (CALC-I gene) is subject to alternative tissue-specific processing of its primary transcript. CT mRNA is the predominant mRNA produced in thyroid C cells, whereas CT gene-related peptide I mRNA is the main product in neurons of the central and peripheral nervous systems. The CT-specific exon 4 is surrounded by weak processing sites. In this study we have investigated whether exon 4 sequences are involved in the tissue-specific selection of the exon 4 splice acceptor site. The results indicate that two separate elements, termed A and B, in the 5' part of exon 4 are required for production of CT-specific RNA. These sequences are located between nucleotides 67 and 88 (A) and nucleotides 117 and 146 (B) relative to the 5' end of exon 4. Variation of the distance between these sequence elements and the 3' splice site of exon 4 does not change the processing choice. These sequence elements are functionally equivalent. CT-specific splicing requires the presence of both sequence A and B or duplicates of either sequence element in exon 4. The effect of these sequences on the RNA processing choice is overruled by mutation of the CT-specific uridine branch acceptor nucleotide into a commonly preferred adenosine residue.
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