ArticleMolecular and cellular biology1994
A simple and efficient procedure for generating stable expression libraries by cDNA cloning in a retroviral vector.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 75 citations in OpenAlex.
- In vivo functional screening for systems-level integrative cancer genomics.Nature reviews. Cancer · 2020Review
- SAMSN1 is a tumor suppressor gene in multiple myeloma.Neoplasia (New York, N.Y.) · 2014Article
- Construction of a high-performance human fetal liver-derived lentiviral cDNA library.Molecular and cellular biochemistry · 2008Article
- Article
- Isolation of genes controlling apoptosis through their effects on cell survival.Gene therapy & molecular biology · 2006Article
- High-efficiency system for the construction of adenovirus vectors and its application to the generation of representative adenovirus-based cDNA expression libraries.Journal of virology · 2006Article
- Identification of immature podocyte specific antigen using retrovirus-mediated gene transfer and cell sorting.Clinical and experimental nephrology · 2005Article
- Regulation of apoptosis by fau revealed by functional expression cloning and antisense expression.Oncogene · 2004Article
- The use of gene function to identify the rate-limiting steps controlling cell fate.Cancer immunology, immunotherapy : CII · 2004Review
- Article
- Novel retroviral vectors to facilitate expression screens in mammalian cells.Nucleic acids research · 2002Article
- Functional cloning using pFB retroviral cDNA expression libraries.Molecular biotechnology · 2002Article
- Retrovirus-mediated transfer of prothymosin gene inhibits tumor growth and prolongs survival in murine bladder cancer.Gene therapy · 2001Article
- A method to identify cDNAs based on localization of green fluorescent protein fusion products.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Selection of functional tRNA primers and primer binding site sequences from a retroviral combinatorial library: identification of new functional tRNA primers in murine leukemia virus replication.Nucleic acids research · 2000Article
- Isoforms of c-KIT differ in activation of signalling pathways and transformation of NIH3T3 fibroblasts.Oncogene · 1999Article
- Twist is a potential oncogene that inhibits apoptosis.Genes & development · 1999Article
- Mutational analyses of the SOCS proteins suggest a dual domain requirement but distinct mechanisms for inhibition of LIF and IL-6 signal transduction.The EMBO journal · 1999Article
- Molecular cloning reveals that the p160 Myb-binding protein is a novel, predominantly nucleolar protein which may play a role in transactivation by Myb.Molecular and cellular biology · 1998Article
- Efficient recovery and regeneration of integrated retroviruses.Nucleic acids research · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
cDNA expression cloning is a powerful method for the rescue and identification of genes that are able to confer a readily identifiable phenotype on specific cell types. Retroviral vectors provide several advantages over DNA-mediated gene transfer for the introduction of expression libraries into eukaryotic cells since they can be used to express genes in a wide range of cell types, including those that form important experimental systems such as the hemopoietic system. We describe here a straightforward and efficient method for generating expression libraries by using a murine retroviral vector. Essentially, the method involves the directional cloning of cDNA into the retroviral vector and the generation of pools of stable ecotropic virus producing cells from this DNA. The cells so derived constitute the library, and the virus they yield is used to infect appropriate target cells for subsequent functional screening. We have demonstrated the feasibility of this procedure by constructing several large retroviral libraries (10(5) to 10(6) individual clones) and then using one of these libraries to isolate cDNAs for interleukin-3 and granulocyte-macrophage colony-stimulating factor on the basis of the ability of these factors to confer autonomous growth on the factor-dependent hemopoietic cell line FDC-P1. Moreover, the frequency at which these factor-independent clones were isolated approximated the frequency at which they were represented in the original plasmid library. These results suggest that expression cloning with retroviruses is a practical and efficient procedure and should be a valuable method for the isolation of important regulatory genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.