ArticleMolecular and cellular biology1994
Polypurine sequences within a downstream exon function as a splicing enhancer.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
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105 citing papers in PubMed, 220 citations in OpenAlex.
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- Identification of Cryptic Novel α-Galactosidase A Gene Mutations: Abnormal mRNA Splicing and Large Deletions.JIMD reports · 2016Article
- Improvement of HIV-1 and Human T Cell Lymphotropic Virus Type 1 Replication-Dependent Vectors via Optimization of Reporter Gene Reconstitution and Modification with Intronic Short Hairpin RNA.Journal of virology · 2015Article
- A novel splicing silencer generated by DMD exon 45 deletion junction could explain upstream exon 44 skipping that modifies dystrophinopathy.Journal of human genetics · 2014Article
- Genome-wide analysis of light-regulated alternative splicing mediated by photoreceptors in Physcomitrella patens.Genome biology · 2014Article
- The evolution, impact and properties of exonic splice enhancers.Genome biology · 2013Article
- Homologous SV40 RNA trans-splicing: a new mechanism for diversification of viral sequences and phenotypes.RNA biology · 2013Article
- Evidence for deep phylogenetic conservation of exonic splice-related constraints: splice-related skews at exonic ends in the brown alga Ectocarpus are common and resemble those seen in humans.Genome biology and evolution · 2013Article
- An exon splice enhancer primes IGF2:IGF2R binding site structure and function evolution.Science (New York, N.Y.) · 2012Article
- YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts.Nucleic acids research · 2012Article
- Juxtaposition of two distant, serine-arginine-rich protein-binding elements is required for optimal polyadenylation in Rous sarcoma virus.Journal of virology · 2011Article
- Phosphorylation switches the general splicing repressor SRp38 to a sequence-specific activator.Nature structural & molecular biology · 2008Article
- Negative and positive mRNA splicing elements act competitively to regulate human immunodeficiency virus type 1 vif gene expression.Journal of virology · 2008Article
- Multisite and bidirectional exonic splicing enhancer in CD44 alternative exon v3.RNA (New York, N.Y.) · 2007Article
45 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously shown that a purine-rich sequence located within exon M2 of the mouse immunoglobulin mu gene functions as a splicing enhancer, as judged by its ability to stimulate splicing of a distant upstream intron. This sequence element has been designated ERS (exon recognition sequence). In this study, we investigated the stimulatory effects of various ERS-like sequences, using the in vitro splicing system with HeLa cell nuclear extracts. Here, we show that purine-rich sequences of several natural exons that have previously been shown to be required for splicing function as a splicing enhancer like the ERS of the immunoglobulin mu gene. Moreover, even synthetic polypurine sequences had stimulatory effects on the upstream splicing. Evaluation of the data obtained from the analyses of both natural and synthetic purine-rich sequences shows that (i) alternating purine sequences can stimulate splicing, while poly(A) or poly(G) sequences cannot, and (ii) the presence of U residues within the polypurine sequence greatly reduces the level of stimulation. Competition experiments strongly suggest that the stimulatory effects of various purine-rich sequences are mediated by the same trans-acting factor(s). We conclude from these results that the purine-rich sequences that we examined in this study also represent examples of ERS. Thus, ERS is considered a general splicing element that is present in various exons and plays an important role in splice site selection.
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