ArticleMolecular and cellular biology1994
Interactions among three proteins that specifically activate translation of the mitochondrial COX3 mRNA in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 76 citations in OpenAlex.
- Article
- Mechanisms and regulation of protein synthesis in mitochondria.Nature reviews. Molecular cell biology · 2021Review
- The Cox1 C-terminal domain is a central regulator of cytochromeThe Journal of biological chemistry · 2017Article
- A Novel Function of Pet54 in Regulation of Cox1 Synthesis in Saccharomyces cerevisiae Mitochondria.The Journal of biological chemistry · 2016Article
- Review
- Mitochondrial protein synthesis, import, and assembly.Genetics · 2012Article
- Biogenesis and assembly of eukaryotic cytochrome c oxidase catalytic core.Biochimica et biophysica acta · 2012Review
- A shared RNA-binding site in the Pet54 protein is required for translational activation and group I intron splicing in yeast mitochondria.Nucleic acids research · 2008Article
- Aberrant translation of cytochrome c oxidase subunit 1 mRNA species in the absence of Mss51p in the yeast Saccharomyces cerevisiae.Molecular biology of the cell · 2007Article
- Article
- Search for protein partners of mitochondrial single-stranded DNA-binding protein Rim1p using a yeast two-hybrid system.Folia microbiologica · 2003Article
- Interactions among COX1, COX2, and COX3 mRNA-specific translational activator proteins on the inner surface of the mitochondrial inner membrane of Saccharomyces cerevisiae.Molecular biology of the cell · 2003Article
- Self-association and mapping of the interaction domain of hepatitis E virus ORF3 protein.Journal of virology · 2001Article
- Article
- Molecular cloning of the maize gene crp1 reveals similarity between regulators of mitochondrial and chloroplast gene expression.The EMBO journal · 1999Article
- Article
- Accumulation of mitochondrially synthesized Saccharomyces cerevisiae Cox2p and Cox3p depends on targeting information in untranslated portions of their mRNAs.The EMBO journal · 1998Article
- Translation of the chloroplast psbA mRNA requires the nuclear-encoded poly(A)-binding protein, RB47.The Journal of cell biology · 1998Article
- Interactions among Drosophila nuclear envelope proteins lamin, otefin, and YA.Molecular and cellular biology · 1998Article
- The ATP-dependent PIM1 protease is required for the expression of intron-containing genes in mitochondria.Genes & development · 1998Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The PET54, PET122, and PET494 proteins, which are associated with the yeast inner mitochondrial membrane, specifically activate translation of the mitochondrially encoded COX3 mRNA. We used the two-hybrid system to test whether pairs of these proteins, when fused to either the GAL4 DNA-binding or transcriptional activating domain, can physically associate as measured by the expression of the GAL4-dependent reporter, lacZ. PET54 and PET122 interacted in this system, and an amino-terminally truncated PET494 fragment showed an interaction with PET54. We also detected functional interactions between PET54 and PET122 genetically: a pet54 missense substitution (Phe to Gly at position 244) that caused a severe respiratory defect was suppressed both by a missense substitution affecting PET122 (Gly to Val at position 211) and by overproduction of wild-type PET122. Both Gly and Ala, substituted at PET54 position 244, disrupted the two-hybrid interactions with PET122 and PET494. While Ala at PET54 position 244 caused only a modest respiratory phenotype alone, it caused a severe respiratory defect when combined with a cold-sensitive mitochondrial mutation affecting the COX3 mRNA 5' leader. This synthetic defect was suppressed by a missense substitution in PET122 and by overproduction of wild-type PET122, indicating functional interactions among PET54, PET122, and the mRNA. Taken together with previous work, these data suggest that a complex containing PET54, PET122, and PET494 mediates the interaction of the COX3 mRNA with mitochondrial ribosomes at the surface of the inner membrane.
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