ArticleJournal of virology1994
A baculovirus gp64 early promoter is activated by host transcription factor binding to CACGTG and GATA elements.
Article in Journal of virology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 80 citations in OpenAlex.
- Productivity and genetic stability of a novel baculovirus vector for multigene expression from independent transgene loci.Molecular therapy. Advances · 2026Article
- NezhNPV, a new biocontrol agent for Nesodiprion zhejiangensis Zhou & Xiao (Hymenoptera: Diprionidae), an emerging forest pest.Pest management science · 2025Article
- Comparative genomics of the Neodiprion sertifer nucleopolyhedrovirus from Turkey with the fewest ORFs among baculoviruses.Virus genes · 2024Article
- Entomopathogenic Viruses in the Neotropics: Current Status and Recently Discovered Species.Neotropical entomology · 2020Review
- Host AAA+ ATPase TER94 Plays Critical Roles in Building the Baculovirus Viral Replication Factory and Virion Morphogenesis.Journal of virology · 2020Article
- Article
- A betabaculovirus encoding a gp64 homolog.BMC genomics · 2016Article
- Characterization of a late gene, ORF75 from Bombyx mori nucleopolyhedrovirus.Molecular biology reports · 2011Article
- Functional and structural characterisation of AgMNPV ie1.Virus genes · 2007Article
- Identification and characterization of odv-e25 of Spodoptera litura multicapsid nucleopolyhedrovirus.Virus genes · 2006Article
- Cloning and sequence analysis of the Antheraea pernyi nucleopolyhedrovirus gp64 gene.Journal of biosciences · 2005Article
- Characterization of p24 gene of Spodoptera litura multicapsid nucleopolyhedrovirus.Virus genes · 2005Article
- Sequence analysis of the genome of the Neodiprion sertifer nucleopolyhedrovirus.Journal of virology · 2004Article
- Molecular cloning and sequence analysis of the Anticarsia gemmatalis multicapsid nuclear polyhedrosis virus GP64 glycoprotein.Virus genes · 2003Article
- Identification of a novel protein associated with envelope of occlusion-derived virus in Spodoptera litura multicapsid nucleopolyhedrovirus.Virus genes · 2003Article
- A discrete stage of baculovirus GP64-mediated membrane fusion.Molecular biology of the cell · 1999Article
- The LEF-4 subunit of baculovirus RNA polymerase has RNA 5'-triphosphatase and ATPase activities.Journal of virology · 1998Article
- The p95 gene of Bombyx mori nuclear polyhedrosis virus: temporal expression and functional properties.Journal of virology · 1998Article
- Identification of two independent transcriptional activation domains in the Autographa californica multicapsid nuclear polyhedrosis virus IE1 protein.Journal of virology · 1997Article
- Baculovirus gp64 gene expression: negative regulation by a minicistron.Journal of virology · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The early promoter of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus gp64 gene is active when transfected into several insect cell lines and does not require viral gene products for transcription in uninfected cells. Because previous studies have shown that the gp64 early promoter is activated above basal levels in uninfected cells, host transcription factors are likely to play a role in gp64 activation at early times postinfection. By using nuclear extracts from uninfected Sf9 cells for electrophoretic mobility shift analysis of gp64 regulatory regions, host nuclear proteins were shown to bind specifically to the upstream regulatory region of the gp64 early promoter. Host factor binding was mapped to a 24-bp sequence centered approximately 35 bp upstream of the TATA box. Two consensus eukaryotic transcription factor-binding site motifs, GATA and CACGTG, were identified within the 24-bp sequence. Competition assays using oligonucleotides containing either a GATA or a CACGTG motif and similar oligonucleotides with point mutations in these sites showed that each site is required for binding host transcription factors. To investigate the functional significance of host factor binding to GATA and CACGTG motifs, constructs containing point mutations in these motifs were examined in transient expression assays. Mutations in either or both GATA and CACGTG sites decreased reporter activity in transient expression assays, suggesting that binding of host transcription factors to these motifs is important in transcriptional regulation of the gp64 early promoter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.