ArticleJournal of bacteriology1994
Purification and properties of a membrane-bound lytic transglycosylase from Escherichia coli.
Article in Journal of bacteriology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- The lytic transglycosylase MltA participates in turnover of septal peptidoglycan inbioRxiv : the preprint server for biology · 2026Article
- A novel peptidoglycan deacetylase modulates daughter cell separation in E. coli.PLoS genetics · 2025Article
- The DigH glycosyl hydrolase is conditionally required for daughter cell separation inJournal of bacteriology · 2025Article
- Recent advances in understanding of enterobacterial common antigen synthesis and regulation.Open biology · 2025Review
- The active repertoire of Escherichia coli peptidoglycan amidases varies with physiochemical environment.Molecular microbiology · 2021Article
- Review
- AFrontiers in microbiology · 2019Article
- Attention Seeker: Production, Modification, and Release of Inflammatory Peptidoglycan Fragments in Neisseria Species.Journal of bacteriology · 2017Review
- Lytic transglycosylases: concinnity in concision of the bacterial cell wall.Critical reviews in biochemistry and molecular biology · 2017Review
- The bacterial septal ring protein RlpA is a lytic transglycosylase that contributes to rod shape and daughter cell separation in Pseudomonas aeruginosa.Molecular microbiology · 2014Article
- Peptidoglycan hydrolases of Escherichia coli.Microbiology and molecular biology reviews : MMBR · 2011Review
- Cell wall hydrolases affect germination, vegetative growth, and sporulation in Streptomyces coelicolor.Journal of bacteriology · 2009Article
- Growth of Escherichia coli: significance of peptidoglycan degradation during elongation and septation.Journal of bacteriology · 2008Article
- The C-terminal domain of Escherichia coli YfhD functions as a lytic transglycosylase.The Journal of biological chemistry · 2008Article
- The Inactivation of a New Peptidoglycan Hydrolase Pmp23 Leads to Abnormal Septum Formation in Streptococcus pneumoniae.The open microbiology journal · 2008Article
- A Drosophila pattern recognition receptor contains a peptidoglycan docking groove and unusual L,D-carboxypeptidase activity.PLoS biology · 2004Article
- The lytic enzyme of bacteriophage PRD1 is associated with the viral membrane.Journal of bacteriology · 2002Article
- Linkage map of Escherichia coli K-12, edition 10: the traditional map.Microbiology and molecular biology reviews : MMBR · 1998Review
- Membrane-bound lytic endotransglycosylase in Escherichia coli.Journal of bacteriology · 1998Article
- Temperature sensitivity of bacteriolysis induced by beta-lactam antibiotics in amino acid-deprived Escherichia coli.Journal of bacteriology · 1998Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A membrane-bound lytic transglycosylase (Mlt) has been solubilized in the presence of 2% Triton X-100 containing 0.5 M NaCl from membranes of an Escherichia coli mutant that carries a deletion in the slt gene coding for a 70-kDa soluble lytic transglycosylase (Slt70). The enzyme was purified by a four-step procedure including anion-exchange (HiLoad SP-Sepharose and MonoS), heparin-Sepharose, and poly(U)-Sepharose 4B column chromatography. The purified protein that migrated during denaturing sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a single band corresponding to an apparent molecular mass of about 38 kDa is referred to as Mlt38. Optimal activity was found in buffers with a pH between 4.0 and 4.5. The enzyme is stimulated by a factor of 2.5 in the presence of Mg2+ at a concentration of 10 mM and loses its activity rapidly at temperatures above 30 degrees C. Besides insoluble murein sacculi, the enzyme was able to degrade glycan strands isolated from murein by amidase treatment. The enzymatic reaction occurred with a maximal velocity of about 2.2 mg/liter/min with murein sacculi as a substrate. The amino acid sequences of four proteolytic peptides showed no identity with known sequences in the data bank. With Mlt38, the number of proteins in E. coli showing lytic transglycosylase activity rises to three.
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