ArticleNucleic acids research1993
Methylation profiles of genomic DNA of mouse developmental brain detected by restriction landmark genomic scanning (RLGS) method.
Article in Nucleic acids research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 110 citations in OpenAlex.
- DNA methylation methods: Global DNA methylation and methylomic analyses.Methods (San Diego, Calif.) · 2021Review
- Physiological and pathological implications of 5-hydroxymethylcytosine in diseases.Oncotarget · 2016Review
- Article
- Identification of DNA methylation changes at cis-regulatory elements during early steps of HSC differentiation using tagmentation-based whole genome bisulfite sequencing.Cell cycle (Georgetown, Tex.) · 2014Article
- Identification of a novel methylated gene in nasopharyngeal carcinoma: TTC40.BioMed research international · 2014Article
- Genome-wide survey reveals dynamic widespread tissue-specific changes in DNA methylation during development.BMC genomics · 2011Article
- QDMR: a quantitative method for identification of differentially methylated regions by entropy.Nucleic acids research · 2011Article
- DNA methylation: a timeline of methods and applications.Frontiers in genetics · 2011Article
- Experimental approaches to the study of epigenomic dysregulation in ageing.Experimental gerontology · 2010Review
- Principles and challenges of genomewide DNA methylation analysis.Nature reviews. Genetics · 2010Review
- Tissue-specific variation in DNA methylation levels along human chromosome 1.Epigenetics & chromatin · 2009Article
- Tissue specific differentially methylated regions (TDMR): Changes in DNA methylation during development.Genomics · 2009Article
- Article
- Aberrant methylations in cancer cells: where do they come from?Cancer science · 2005Review
- Article
- Non-methylated Genomic Sites Coincidence Cloning (NGSCC): an approach to large scale analysis of hypomethylated CpG patterns at predetermined genomic loci.Molecular genetics and genomics : MGG · 2004Article
- Aberrant methylation of the vascular endothelial growth factor receptor-1 gene in prostate cancer.Cancer science · 2003Article
- Review
- A novel technique for the identification of CpG islands exhibiting altered methylation patterns (ICEAMP).Nucleic acids research · 2001Article
- Restriction landmark cDNA scanning (RLCS): a novel cDNA display system using two-dimensional gel electrophoresis.Nucleic acids research · 1996Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Restriction landmark genomic scanning using methylation-sensitive endonucleases (RLGS-M) is a newly developed powerful method for systematic detection of DNA methylation. Using this method, we scanned mouse brain genomic DNAs from various developmental stages to detect the transcriptionally active regions. This approach is based on the assumption that CpG methylation, particularly of CpG islands, might be associated with gene transcriptional regulation. Genomic DNAs were prepared from telencephalons of 9.5-, 13.5- and 16.5-day embryos, 1- and 10-day neonates and adults, followed by subjecting them to RLGS-M and comparing their patterns with each other or with that of the adult liver. We used NotI as a methylation-sensitive restriction enzyme and surveyed the methylation states of 2,600 NotI sites, almost of which should correspond to gene loci. Although almost all RLGS spots (98%) were present constantly at every developmental stages, only a few percent of spots reproducibly appeared and disappeared at different developmental stages of the brain (44 spots, 1.7%) and some were tissue-specific (10 spots, 0.7%). These data suggest that DNA methylation associated with gene transcription is a well-programmed event during the central nervous system (CNS) development. Thus, RLGS-M can offer a means for detecting systematically the genes in which the state of DNA methylation changes during development of the higher organism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.