ArticleThe Biochemical journal1994
Phospholipase C in Dictyostelium discoideum. Cyclic AMP surface receptor and G-protein-regulated activity in vitro.
Article in The Biochemical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- Biochemical and genetic evidence for the presence of multiple phosphatidylinositol- and phosphatidylinositol 4,5-bisphosphate-specific phospholipases C in Tetrahymena.Eukaryotic cell · 2011Article
- Oscillatory signaling and network responses during the development of Dictyostelium discoideum.Ageing research reviews · 2008Review
- Phospholipase C regulation of phosphatidylinositol 3,4,5-trisphosphate-mediated chemotaxis.Molecular biology of the cell · 2007Article
- Chemoattractants and chemorepellents act by inducing opposite polarity in phospholipase C and PI3-kinase signaling.The Journal of cell biology · 2007Article
- A Dictyostelium nuclear phosphatidylinositol phosphate kinase required for developmental gene expression.The EMBO journal · 2001Article
- Phospholipase Cdelta regulates germination of Dictyostelium spores.BMC cell biology · 2001Article
- Ethanol and acetaldehyde elevate intracellular [Ca2+] and stimulate microneme discharge in Toxoplasma gondii.The Biochemical journal · 1999Article
- Fatty acids induce release of Ca2+ from acidosomal stores and activate capacitative Ca2+ entry in Dictyostelium discoideum.The Biochemical journal · 1998Article
- The Dictyostelium MAP kinase kinase DdMEK1 regulates chemotaxis and is essential for chemoattractant-mediated activation of guanylyl cyclase.The EMBO journal · 1997Article
- The role of calcium in aggregation and development of Dictyostelium.Experientia · 1995Review
- Transduction of the chemotactic cAMP signal across the plasma membrane of Dictyostelium cells.Experientia · 1995Review
- Mutation of an EF-hand Ca(2+)-binding motif in phospholipase C of Dictyostelium discoideum: inhibition of activity but no effect on Ca(2+)-dependence.The Biochemical journal · 1995Article
- In Dictyostelium discoideum inositol 1,3,4,5-tetrakisphosphate is dephosphorylated by a 3-phosphatase and a 1-phosphatase.The Biochemical journal · 1995Article
- A model for cAMP-mediated cGMP response in Dictyostelium discoideum.Molecular biology of the cell · 1994Article
- Role of phospholipase C in Dictyostelium: formation of inositol 1,4,5-trisphosphate and normal development in cells lacking phospholipase C activity.The EMBO journal · 1994Article
- Phospholipase C in Dictyostelium discoideum. Identification of stimulatory and inhibitory surface receptors and G-proteins.The Biochemical journal · 1994Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cellular slime mould Dictyostelium discoideum shows several responses after stimulation with the chemoattractant cAMP, including a transient rise in cyclic AMP (cAMP), cGMP and Ins(1,4,5)P3. In this paper the regulation of phospholipase C in vitro is described. Under our experimental conditions commercial PtdIns(4,5)P2 cannot be used to analyse phospholipase C activity in Dictyostelium lysates, because it is hydrolysed mainly to glycerophosphoinositol instead of Ins(1,4,5)P3. Enzyme activity was determined with endogenous unlabelled PtdInsP2 as a substrate. The product was measured by isotope-dilution assay and identified as authentic Ins(1,4,5)P3. Since phospholipase C is strictly Ca(2+)-dependent, with an optimal concentration range of 1-100 microM, cell lysates were prepared in EGTA and the enzyme reaction was started by adding 10 microM free Ca2+. Phospholipase C activity increased 2-fold during Dictyostelium development up to 8 h of starvation, after which the activity declined to less than 10% of the vegetative level. Enzyme activity in vitro increased up to 2-fold after stimulation of cells with the agonist cAMP in vivo. Addition of 10 microM guanosine 5'-[gamma-thio]triphosphate during lysis activated the enzyme to the same extent, and this effect was antagonized by guanosine 5'-[beta-thio]diphosphate. These results strongly suggest that surface cAMP receptors and G-proteins regulate phospholipase C during Dictyostelium development.
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