ArticleThe Biochemical journal1993
Calponin phosphorylation in vitro and in intact muscle.
Article in The Biochemical journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 92 citations in OpenAlex.
- Mechanoregulation and function of calponin and transgelin.Biophysics reviews · 2024Review
- A Preparative Method for the Isolation of Calponin from Molluscan Catch Muscle.International journal of molecular sciences · 2022Article
- A rapid degradation of calponin 2 is required for cytokinesis.American journal of physiology. Cell physiology · 2021Article
- Myosin Rod Hypophosphorylation and CB Kinetics in Papillary Muscles from a TnC-A8V KI Mouse Model.Biophysical journal · 2017Article
- Deletion of calponin 2 in macrophages attenuates the severity of inflammatory arthritis in mice.American journal of physiology. Cell physiology · 2016Article
- Review
- Mechanisms of Vascular Smooth Muscle Contraction and the Basis for Pharmacologic Treatment of Smooth Muscle Disorders.Pharmacological reviews · 2016Review
- Smooth Muscle Stiffness Sensitivity is Driven by Soluble and Insoluble ECM Chemistry.Cellular and molecular bioengineering · 2015Article
- Mechanoregulation of h2-calponin gene expression and the role of Notch signaling.The Journal of biological chemistry · 2014Article
- Unphosphorylated calponin enhances the binding force of unphosphorylated myosin to actin.Biochimica et biophysica acta · 2013Article
- A multi-scale approach to airway hyperresponsiveness: from molecule to organ.Frontiers in physiology · 2012Article
- The calponin regulatory region is intrinsically unstructured: novel insight into actin-calponin and calmodulin-calponin interfaces using NMR spectroscopy.Biophysical journal · 2011Article
- Phosphorylated HSP20 modulates the association of thin-filament binding proteins: caldesmon with tropomyosin in colonic smooth muscle.American journal of physiology. Gastrointestinal and liver physiology · 2010Article
- Maturation and the role of PKC-mediated contractility in ovine cerebral arteries.American journal of physiology. Heart and circulatory physiology · 2009Article
- Calponin control of cerebrovascular reactivity: therapeutic implications in brain trauma.Journal of cellular and molecular medicine · 2009Review
- Smooth muscle signalling pathways in health and disease.Journal of cellular and molecular medicine · 2008Review
- Role of H2-calponin in regulating macrophage motility and phagocytosis.The Journal of biological chemistry · 2008Article
- Calponin (CaP) as a latch-bridge protein--a new concept in regulation of contractility in smooth muscles.Journal of muscle research and cell motility · 2004Review
- The Saccharomyces cerevisiae calponin/transgelin homolog Scp1 functions with fimbrin to regulate stability and organization of the actin cytoskeleton.Molecular biology of the cell · 2003Article
- Calponin is required for agonist-induced signal transduction--evidence from an antisense approach in ferret smooth muscle.The Journal of physiology · 2001Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Calponin, a thin-filament-associated protein implicated in the regulation of smooth-muscle contraction, is phosphorylated in vitro by protein kinase C and Ca2+/calmodulin-dependent protein kinase II [Winder and Walsh (1990) J. Biol. Chem. 265, 10148-10155] and dephosphorylated by a type 2A protein phosphatase [Winder, Pato and Walsh (1992) Biochem. J. 286, 197-203]. Unphosphorylated calponin binds to actin and inhibits the actin-activated myosin MgATPase; these properties are lost on phosphorylation. Although both serine and threonine residues in calponin are phosphorylated, the major site of phosphorylation by either kinase is Ser-175. Calponin also undergoes phosphorylation when bound to actin in synthetic thin filaments, in a reconstituted actomyosin system, in washed myofibrils and in tissue extracts; this results in dissociation of calponin from actin. Tryptic phosphopeptide mapping indicates that the same sites are phosphorylated in the bound as in the isolated protein. Toad stomach calponin exists in at least three isoforms which differ in charge but exhibit the same molecular mass on SDS/PAGE. In a toad stomach extract, all three isoforms are phosphorylated by protein kinase C or Ca2+/calmodulin-dependent protein kinase II as shown by two-dimensional gel electrophoresis (non-equilibrium pH-gradient gel electrophoresis and SDS/PAGE). Calponin phosphorylation also occurs in intact toad stomach smooth-muscle strips metabolically labelled with 32Pi and stimulated to contract with carbachol. These results support the hypothesis that calponin may be regulated in vivo by phosphorylation-dephosphorylation.
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Registered trials
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