ArticleProceedings of the National Academy of Sciences of the United States of America1994
Construction of libraries enriched for sequence repeats and jumping clones, and hybridization selection for region-specific markers.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
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- Registry in a tube: multiplexed pools of retrievable parts for genetic design space exploration.Nucleic acids research · 2017Article
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- Capturing Darwin's dream.Molecular ecology resources · 2016Article
- Microsatellites in Pursuit of Microbial Genome Evolution.Frontiers in microbiology · 2015Review
- Rapid microsatellite development for tree peony and its implications.BMC genomics · 2013Article
- Managing diversity: Domestication and gene flow in Stenocereus stellatus Riccob. (Cactaceae) in Mexico.Ecology and evolution · 2013Article
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- SSR-patchwork: An optimized protocol to obtain a rapid and inexpensive SSR library using first-generation sequencing technology.Applications in plant sciences · 2013Article
- High-throughput novel microsatellite marker of faba bean via next generation sequencing.BMC genomics · 2012Article
- Microsatellite isolation and marker development in carrot - genomic distribution, linkage mapping, genetic diversity analysis and marker transferability across Apiaceae.BMC genomics · 2011Article
- A modified enrichment method to construct microsatellite library from plateau pika genome (Ochotona curzoniae).Genomics, proteomics & bioinformatics · 2010Article
- Isolation and characterization of microsatellite loci in the western long-fingered bat, Miniopterus magnater.Molecular ecology resources · 2008Article
- Isolation and characterization of polymorphic microsatellite markers for the masked palm civet (Paguma larvata).Biochemical genetics · 2008Article
- Characterization and transferability of microsatellite markers of the cultivated peanut (Arachis hypogaea).BMC plant biology · 2007Article
- Characterization of microsatellite loci in fulvous fruit batMolecular ecology notes · 2006Article
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3 authors.
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Abstract
We describe a simple and rapid method for constructing small-insert genomic libraries highly enriched for dimeric, trimeric, and tetrameric nucleotide repeat motifs. The approach involves use of DNA inserts recovered by PCR amplification of a small-insert sonicated genomic phage library or by a single-primer PCR amplification of Mbo I-digested and adaptor-ligated genomic DNA. The genomic DNA inserts are heat denatured and hybridized to a biotinylated oligonucleotide. The biotinylated hybrids are retained on a Vectrex-avidin matrix and eluted specifically. The eluate is PCR amplified and cloned. More than 90% of the clones in a library enriched for (CA)n microsatellites with this approach contained clones with inserts containing CA repeats. We have also used this protocol for enrichment of (CAG)n and (AGAT)n sequence repeats and for Not I jumping clones. We have used the enriched libraries with an adaptation of the cDNA selection method to enrich for repeat motifs encoded in yeast artificial chromosomes.
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