ArticleProceedings of the National Academy of Sciences of the United States of America1994
Saturation mutagenesis of a polyadenylation signal reveals a hexanucleotide element essential for mRNA 3' end formation in Saccharomyces cerevisiae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 59 citations in OpenAlex.
- Activation of the Endonuclease that Defines mRNA 3' Ends Requires Incorporation into an 8-Subunit Core Cleavage and Polyadenylation Factor Complex.Molecular cell · 2019Article
- Budding yeast telomerase RNA transcription termination is dictated by the Nrd1/Nab3 non-coding RNA termination pathway.Nucleic acids research · 2012Article
- Novel protein-protein contacts facilitate mRNA 3'-processing signal recognition by Rna15 and Hrp1.Journal of molecular biology · 2010Article
- Statistical analysis of yeast genomic downstream sequences reveals putative polyadenylation signals.Nucleic acids research · 2000Article
- Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis.Microbiology and molecular biology reviews : MMBR · 1999Review
- Arginine methylation and binding of Hrp1p to the efficiency element for mRNA 3'-end formation.RNA (New York, N.Y.) · 1999Article
- Nascent transcription from the nmt1 and nmt2 genes of Schizosaccharomyces pombe overlaps neighbouring genes.The EMBO journal · 1998Article
- Transcription termination downstream of the Saccharomyces cerevisiae FBP1 [changed from FPB1] poly(A) site does not depend on efficient 3'end processing.RNA (New York, N.Y.) · 1998Article
- Cloning and characterization of two rhamnogalacturonan hydrolase genes from Aspergillus niger.Applied and environmental microbiology · 1997Article
- Characterization of the pH signal transduction pathway gene palA of Aspergillus nidulans and identification of possible homologs.Journal of bacteriology · 1997Article
- Sequence requirements of the bidirectional yeast TRP4 mRNA 3'-end formation signal.Nucleic acids research · 1997Article
- Functional mapping of the translation-dependent instability element of yeast MATalpha1 mRNA.Molecular and cellular biology · 1996Article
- Yeast retrotransposon Ty4: the majority of the rare transcripts lack a U3-R sequence.Nucleic acids research · 1996Article
- Signals sufficient for 3'-end formation of yeast mRNA.Molecular and cellular biology · 1996Article
- 3'-end-forming signals of yeast mRNA.Molecular and cellular biology · 1995Article
- Cloning, sequence and expression of the gene coding for rhamnogalacturonase of Aspergillus aculeatus; a novel pectinolytic enzyme.Applied microbiology and biotechnology · 1995Article
- Redundant 3' end-forming signals for the yeast CYC1 mRNA.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- A complex unidirectional signal element mediates GCN4 mRNA 3' end formation in Saccharomyces cerevisiae.Molecular and cellular biology · 1995Article
- REF2 encodes an RNA-binding protein directly involved in yeast mRNA 3'-end formation.Molecular and cellular biology · 1995Article
- The Aspergillus PacC zinc finger transcription factor mediates regulation of both acid- and alkaline-expressed genes by ambient pH.The EMBO journal · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cis-acting signal sequences required for mRNA 3' end formation are highly conserved and well characterized in higher eukaryotes. However, the situation in the yeast Saccharomyces cerevisiae is still unclear. Several sequences have been proposed which share only limited similarities. One difficulty in identifying yeast polyadenylylation signals might be the presence of redundant signal sequences in the 3' region of yeast genes. To circumvent this problem we have analyzed the heterologous 3' region from cauliflower mosaic virus which contains a yeast polyadenylylation signal. We have performed a saturation mutagenesis of the key element TAG-TATGTA, which is a condensed version of the polyadenylylation signal TAG ... TATGTA ... (TTT) which had previously been proposed. Each of the nine nucleotides was replaced by the three other possible nucleotides and all resulting 1-bp mutants were tested for their capacity to specify mRNA 3' end formation in yeast cells. The first three nucleotides of this condensed sequence are not required, but mutagenesis of the other six nucleotides had distinct effects on mRNA 3' end formation. All mutants that were significantly functional had the sequence TAYRTA, and the sequence TATATA had the best capacity for mRNA 3' end formation. The two thymidine residues at the first and fifth positions are the most essential nucleotides in this sequence. Our results suggest that a degenerate hexanucleotide is essential for mRNA 3' end formation in yeast. This is reminiscent of the conserved polyadenylylation signal in higher eukaryotes, AATAAA.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.