ArticlePflugers Archiv : European journal of physiology1993
Na/H exchange activities in NHE1-transfected OK-cells: cell polarity and regulation.
Article in Pflugers Archiv : European journal of physiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed.
- The biophysical and molecular basis of intracellular pH sensing by Na+/H+ exchanger-3.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Glucose-induced regulation of NHEs activity and SGLTs expression involves the PKA signaling pathway.The Journal of membrane biology · 2011Article
- The calcineurin homologous protein-1 increases Na(+)/H(+) -exchanger 3 trafficking via ezrin phosphorylation.Journal of the American Society of Nephrology : JASN · 2009Article
- Lipid- and mechanosensitivities of sodium/hydrogen exchangers analyzed by electrical methods.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Inhibition and redistribution of NHE3, the apical Na+/H+ exchanger, by Clostridium difficile toxin B.The Journal of general physiology · 2004Article
- The cytoplasmic domain of the Na+/H+ exchangers (NHEs) dictates the nature of the hormonal response: behavior of a chimeric human NHE1/trout beta NHE antiporter.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Functional adaptation to high PCO2 of apically and basolaterally located Na+/H+ exchange activities in cultured renal cell lines.Pflugers Archiv : European journal of physiology · 1994Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human fibroblast, "amiloride-sensitive" Na/H exchanger (NHE1) was transfected into opossum kidney cells (OK cells) (OK/NHE1 cells). Northern blot analysis confirmed that the NHE1 message is expressed in OK/NHE1 cells. In immunoblot analysis, an anti-human NHE1 antibody labelled a membrane protein only present in OK/NHE1 cells. In contrast to the parental cell line containing only an apically located, "amiloride-resistant" Na/H exchange activity, OK/NHE1 cells contain apically and basolaterally located Na/H exchange activities, the apical activity being "amiloride resistant" and the basolateral being "amiloride sensitive". Parathyroid hormone (PTH) inhibited apical transport activity (OK and OK/NHE1 cells) but had no effect on basolateral transport activity (OK/NHE1 cells). Pharmacological activation of protein kinase A (forskolin) decreased both apical and basolateral Na/H exchange activity. Incubation with phorbol ester (exogenous activation of protein kinase C) reduced apical Na/H exchange activity (OK and OK/NHE1 cells) but had only a moderate, inhibitory effect on basolateral Na/H exchange activity (OK/NHE1 cells). These results indicate that transfection of OK cells with human fibroblast NHE1 cDNA encoding an "amiloride-sensitive" form of the Na/H exchanger results in expression of basolaterally located "NHE1-related" transport activity. Regulatory control of intracellular Na/H exchange activities (apically versus basolaterally located) and intercellular Na/H exchange activities (NHE1-related) differs. This may relate to cell-specific properties as well as to exchanger-specific properties.
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