ArticlePflugers Archiv : European journal of physiology1993
Endothelin and vasopressin activate low conductance chloride channels in aortic smooth muscle cells.
Article in Pflugers Archiv : European journal of physiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 72 citations in OpenAlex.
- Clues and new evidences in arterial hypertension: unmasking the role of the chloride anion.Pflugers Archiv : European journal of physiology · 2022Review
- Molecular mechanisms of activation and regulation of ANO1-Encoded CaChannels (Austin, Tex.) · 2021Review
- Influence of intracellular Ca2+ and alternative splicing on the pharmacological profile of ANO1 channels.American journal of physiology. Cell physiology · 2016Article
- Activation of Ca(2+) -activated Cl(-) channel ANO1 by localized Ca(2+) signals.The Journal of physiology · 2016Review
- Molecular and functional significance of Ca(2+)-activated Cl(-) channels in pulmonary arterial smooth muscle.Pulmonary circulation · 2015Review
- The hidden hand of chloride in hypertension.Pflugers Archiv : European journal of physiology · 2015Review
- TMEM16A knockdown abrogates two different Ca(2+)-activated Cl (-) currents and contractility of smooth muscle in rat mesenteric small arteries.Pflugers Archiv : European journal of physiology · 2014Article
- Cl⁻ channels in smooth muscle cells.Pflugers Archiv : European journal of physiology · 2014Review
- Potent vasorelaxant activity of the TMEM16A inhibitor T16A(inh) -A01.British journal of pharmacology · 2013Article
- Physiological role of inward rectifier K(+) channels in vascular smooth muscle cells.Pflugers Archiv : European journal of physiology · 2008Review
- Vasopressin stimulates action potential firing by protein kinase C-dependent inhibition of KCNQ5 in A7r5 rat aortic smooth muscle cells.American journal of physiology. Heart and circulatory physiology · 2007Article
- Multiple conductance states of single Ca2+-activated Cl- channels in rabbit pulmonary artery smooth muscle cells.The Journal of physiology · 2003Article
- Differential regulation of Ca(2+)-activated Cl(-) currents in rabbit arterial and portal vein smooth muscle cells by Ca(2+)-calmodulin-dependent kinase.The Journal of physiology · 2001Article
- Bimodal control of a Ca(2+)-activated Cl(-) channel by different Ca(2+) signals.The Journal of general physiology · 2000Article
- Modulation of the decay of Ca2+-activated Cl- currents in rabbit portal vein smooth muscle cells by external anions.The Journal of physiology · 1999Article
- Long-term induction of a unique C1- current by endothelin-1 in an epithelial cell line from rat lung: evidence for regulation of cytoplasmic calcium.The Journal of physiology · 1998Article
- Non-specificity of chloride channel blockers in rat cerebral arteries: block of the L-type calcium channel.The Journal of physiology · 1998Article
- Chloride channel blockers inhibit myogenic tone in rat cerebral arteries.The Journal of physiology · 1997Article
- Serum-induced membrane depolarization in quiescent fibroblasts: activation of a chloride conductance through the G protein-coupled LPA receptor.The EMBO journal · 1996Article
- A maxi Cl- channel coupled to endothelin B receptors in the basolateral membrane of guinea-pig parietal cells.The Journal of physiology · 1995Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The non-contractile aortic smooth muscle cell line A7r5 was used to study the membrane events involved in the effect of vasoconstrictor peptides. Whole-cell voltage-clamp and membrane potential recording techniques were used to demonstrate the contribution of an increased Cl- conductance to the late depolarization induced by endothelin-1 and vasopressin. During cell-attached patch recording with N-methyl-D-glucamine in the pipette, bath application of endothelin or vasopressin induced single-channel inward currents in the following minutes. The current/potential (I/V) curve of the most frequently observed channel type--a small conductance Cl- (SCl) channel--reversed near the cell membrane potential and showed a single-channel conductance of 1.8 pS for inward currents. After patch excision in an extracellular solution containing CaCl2 (2 mM), the frequency of SCl channel openings increased. Patch excision in the absence of peptide stimulation also produced this channel activity. Replacement of CaCl2 by a Ca2+ chelator on the intracellular face of a patch reversibly inhibited the channel activity, indicating that these SCl channels are Ca(2+)-activated Cl- channels. The single-channel I/V characteristic showed outward rectification above +50 mV. An analysis of the gating kinetics of the SCl channel is given. Another channel type was recorded less frequently after peptide stimulation. It had a lower conductance (1.0-1.3 pS) and slower kinetics and was designated a very small conductance Cl- channel. It is concluded that activation of two types of Cl- channels (at least one of which is Ca2+ dependent) is involved in the late depolarization produced by vasoconstrictor peptides in vascular smooth muscle cells of the aortic cell line A7r5.
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