ArticlePflugers Archiv : European journal of physiology1993
Synergistic activation of non-rectifying small-conductance chloride channels by forskolin and phorbol esters in cell-attached patches of the human colon carcinoma cell line HT-29cl.19A.
Article in Pflugers Archiv : European journal of physiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 35 citations in OpenAlex.
- Molecular and functional characterization of the cystic fibrosis transmembrane conductance regulator from the Australian common brushtail possum, Trichosurus vulpecula.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2010Article
- Phosphorylation of protein kinase C sites in NBD1 and the R domain control CFTR channel activation by PKA.The Journal of physiology · 2003Article
- Polarized signaling via purinoceptors in normal and cystic fibrosis airway epithelia.The Journal of general physiology · 2001Article
- Purinoceptor-coupled Cl- channels in mouse heart: a novel, alternative pathway for CFTR regulation.The Journal of physiology · 1999Article
- Halide permeation in wild-type and mutant cystic fibrosis transmembrane conductance regulator chloride channels.The Journal of general physiology · 1997Article
- Permeability of wild-type and mutant cystic fibrosis transmembrane conductance regulator chloride channels to polyatomic anions.The Journal of general physiology · 1997Article
- Phorbol ester activation of chloride current in guinea-pig ventricular myocytes.British journal of pharmacology · 1996Article
- Chloride secretion induced by phorbol dibutyrate and forskolin in the human colonic carcinoma cell line HT-29Cl.19A is regulated by different mechanisms.Pflugers Archiv : European journal of physiology · 1995Article
- Characterization of two distinct Cl- conductances in fused human respiratory epithelial cells. I. Anion selectivities, stimulation and intermeshing signal transduction pathways.Pflugers Archiv : European journal of physiology · 1995Article
- Cellular chloride depletion inhibits cAMP-activated electrogenic chloride fluxes in HT29-18-C1 cells.The Journal of membrane biology · 1995Article
- Activation of ion transport by combined effects of ionomycin, forskolin and phorbol ester on cultured HT-29cl.19A human colonocytes.Pflugers Archiv : European journal of physiology · 1993Article
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5 authors at 2 institutions in 1 country.
Funding
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Abstract
Cell-attached patch-clamp studies with the human colon carcinoma HT-29cl.19A cells revealed a small chloride channel with a unitary conductance of 6.5 pS at 70 mV and 4.6 pS at -70 mV clamp potential after cAMP was increased by activation of adenylyl cyclase by forskolin. Usually channels inactivated upon patch excision, but in a few excised patches the channels stayed active and displayed a linear I/V relation in symmetrical (150 mmol/l) chloride solutions with a conductance of 7.5 pS. A 16-fold increase in channel incidence was observed when forskolin and phorbol 12,13-dibutyrate (PDB) were present together. The open probability was voltage-independent and was not different in the presence of forskolin plus PDB or with forskolin alone. The conductance sequence of the channel as deduced from outward currents carried by five different anions including chloride was: Cl- > BR- > NO3- > gluconate > I-. The permeability sequence deduced from the reversal potentials was NO3- > or = Br- > Cl- > I- > gluconate. With iodide in the pipette the conductance decreased strongly. Moreover, the inward current was reduced by 61%, indicating a strong inhibition of the chloride efflux by iodide. Similarly, the forskolin-induced increase of the short-circuit current (Isc) in confluent filter-grown monolayers was strongly reduced by iodide in the apical perfusate. Iodide also increased the fractional resistance of the apical membrane and repolarized the membrane potential, indicating an inhibitory action on the forskolin-induced increase of the apical chloride conductance.(ABSTRACT TRUNCATED AT 250 WORDS)
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