ArticleThe Journal of physiology1993
Calcium currents elicited by voltage steps and steady voltages in myocytes isolated from the rat basilar artery.
Article in The Journal of physiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 49 citations in OpenAlex.
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- Comparison of inhibitory neuromuscular transmission in the Cynomolgus monkey IAS and rectum: special emphasis on differences in purinergic transmission.The Journal of physiology · 2018Article
- Role of T-type channels in vasomotor function: team player or chameleon?Pflugers Archiv : European journal of physiology · 2014Review
- Local regulation of L-type Ca²⁺ channel sparklets in arterial smooth muscle.Microcirculation (New York, N.Y. : 1994) · 2013Review
- T-type calcium channels and vascular function: the new kid on the block?The Journal of physiology · 2011Review
- Voltage-dependent calcium channels of dog basilar artery.The Journal of physiology · 2007Article
- Two Ca2+ entry pathways mediate InsP3-sensitive store refilling in guinea-pig colonic smooth muscle.The Journal of physiology · 2000Article
- Blockade by 18beta-glycyrrhetinic acid of intercellular electrical coupling in guinea-pig arterioles.The Journal of physiology · 1998Article
- Streptomycin inhibition of myogenic tone, K+-induced force and block of L-type calcium current in rat cerebral arteries.The Journal of physiology · 1998Article
- Ryanodine receptors regulate arterial diameter and wall [Ca2+] in cerebral arteries of rat via Ca2+-dependent K+ channels.The Journal of physiology · 1998Article
- Regulation of arterial diameter and wall [Ca2+] in cerebral arteries of rat by membrane potential and intravascular pressure.The Journal of physiology · 1998Article
- Voltage-dependent calcium currents and cytosolic calcium in equine airway myocytes.The Journal of physiology · 1996Article
- Ca2+ currents in cerebral artery smooth muscle cells of rat at physiological Ca2+ concentrations.The Journal of general physiology · 1996Article
- Effects of the BKCa channel activator, NS1619, on rat cerebral artery smooth muscle.British journal of pharmacology · 1996Article
- beta-Adrenoceptor stimulation activates large-conductance Ca2+-activated K+ channels in smooth muscle cells from basilar artery of guinea pig.Pflugers Archiv : European journal of physiology · 1995Article
- Efficacy of peak Ca2+ currents (ICa) as trigger of sarcoplasmic reticulum Ca2+ release in myocytes from the guinea-pig coronary artery.The Journal of physiology · 1995Article
- Voltage window for sustained elevation of cytosolic calcium in smooth muscle cells.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Calcium channel currents recorded from isolated myocytes of rat basilar artery are stretch sensitive.The Journal of physiology · 1993Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
1. Whole-cell patch clamp methods were used to record Ca2+ channel currents from isolated rat basilar arterial myocytes either in response to voltage steps or at steady holding potentials. Inward currents were rapidly and reversibly blocked by 2 mM Co2+, and this solution was routinely used to subtract leakage currents. 2. Peak currents measured in response to voltage steps were -85 +/- 23 pA (n = 7) in physiological Ca2+ (1.8 mM) and -256 +/- 39 pA (n = 19) in 10 mM Ba2+. The time course of activation and inactivation were unaffected by changing the holding potential from -88 to -48 mV, and thus we found no evidence for a component of current flowing through transient Ca2+ channels. 3. Activation curves were constructed by dividing the macroscopic current by the single Ca2+ channel current. The product of the number of functional channels and the open-state probability (NPo) measured in this way rose to around 2000. Its dependence on voltage was fitted by a Boltzmann function with half-activation at -8.6 mV in physiological Ca2+ concentrations and a steepness factor in the range 6-8 mV. 4. In physiological solution, block by external Mg2+ reduced peak current through Ca2+ channels by 17%. 5. Steady-state currents were measured by holding the cell at a fixed voltage and rapidly applying Co2+ to block the current through Ca2+ channels. Steady-state currents could be detected at voltages as negative as -58 mV, and persisted for more than 15 min, suggesting that Ca2+ channels in this artery may provide a steady-state Ca2+ influx that contributes to resting contractile tone.
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