ArticleThe Journal of experimental medicine1994
Enhancer-dependent and -independent steps in the rearrangement of a human T cell receptor delta transgene.
Article in The Journal of experimental medicine, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 82 citations in OpenAlex.
- Epigenetic aspects of lymphocyte antigen receptor gene rearrangement or 'when stochasticity completes randomness'.Immunology · 2013Review
- Orchestrating T-cell receptor α gene assembly through changes in chromatin structure and organization.Immunologic research · 2011Article
- T cell receptor (TCR) alpha/delta locus enhancer identity and position are critical for the assembly of TCR delta and alpha variable region genes.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Article
- Ordered assembly of the V(D)J synaptic complex ensures accurate recombination.The EMBO journal · 2002Article
- Stepwise activation of the immunoglobulin mu heavy chain gene locus.The EMBO journal · 2001Article
- Regulated T-cell development: a victim of multiple conspiracies.Immunology · 2001Review
- Assessing a role for enhancer-blocking activity in gene regulation within the murine T-cell receptor alpha/delta locus.Immunology · 2001Article
- Accessibility control of T cell receptor gene rearrangement in developing thymocytes. The TCR alpha/delta locus.Immunologic research · 2000Review
- Flanking nuclear matrix attachment regions synergize with the T cell receptor delta enhancer to promote V(D)J recombination.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Enhancer control of V(D)J recombination at the TCRbeta locus: differential effects on DNA cleavage and joining.Genes & development · 1998Article
- Cooperation among multiple transcription factors is required for access to minimal T-cell receptor alpha-enhancer chromatin in vivo.Molecular and cellular biology · 1998Article
- Alternative splicing of rearranged T cell receptor delta sequences to the constant region of the alpha locus.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Enhancer control of local accessibility to V(D)J recombinase.Molecular and cellular biology · 1997Article
- An enhancer-blocking element between alpha and delta gene segments within the human T cell receptor alpha/delta locus.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Characterization of excised DNA intermediates associated with V(D)J recombination at the T-cell receptor delta locus.Molecular and cellular biology · 1997Article
- Regulation of T cell receptor delta gene rearrangement by CBF/PEBP2.The Journal of experimental medicine · 1997Article
- A targeted mutation at the T-cell receptor alpha/delta locus impairs T-cell development and reveals the presence of the nearby antiapoptosis gene Dad1.Molecular and cellular biology · 1997Article
- Developmental regulation of VDJ recombination by the core fragment of the T cell receptor alpha enhancer.The Journal of experimental medicine · 1997Article
- Gene-targeted deletion and replacement mutations of the T-cell receptor beta-chain enhancer: the role of enhancer elements in controlling V(D)J recombination accessibility.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The rearrangement and expression of T cell receptor (TCR) gene segments occurs in a highly ordered fashion during thymic ontogeny of T lymphocytes. To study the regulation of gene rearrangement within the TCR alpha/delta locus, we generated transgenic mice that carry a germline human TCR delta minilocus that includes V delta 1, V delta 2, D delta 3, J delta 1, J delta 3, and C delta segments, and either contains or lacks the TCR delta enhancer. We found that the enhancer-positive construct rearranges stepwise, first V to D, and then V-D to J. Construct V-D rearrangement mimics a unique property of the endogenous TCR delta locus. V-D-J rearrangement is T cell specific, but is equivalent in alpha/beta and gamma/delta T lymphocytes. Thus, either there is no commitment to the alpha/beta and gamma/delta T cell lineages before TCR delta gene rearrangement, or if precommitment occurs, it does not operate directly on TCR delta gene cis-acting regulatory elements to control TCR delta gene rearrangement. Enhancer-negative mice display normal V to D rearrangement, but not V-D to J rearrangement. Thus, the V-D to J step is controlled by the enhancer, but the V to D step is controlled by separate elements. The enhancer apparently controls access to J delta 1 but not D delta 3, suggesting that a boundary between two independently regulated domains of the minilocus lies between these elements. Within the endogenous TCR alpha/delta locus, this boundary may represent the 5' end of a chromatin regulatory domain that is opened by the TCR delta enhancer during T cell development. The position of this boundary may explain the unique propensity of the TCR delta locus to undergo early V to D rearrangement. Our results indicate that the TCR delta enhancer performs a crucial targeting function to regulate TCR delta gene rearrangement during T cell development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.