ArticleNucleic acids research1993
Identification of positive and negative regulatory elements involved in the retinoic acid/cAMP induction of Fgf-3 transcription in F9 cells.
Article in Nucleic acids research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 21 citations in OpenAlex.
- Retinoids: Mechanisms of Action in Neuronal Cell Fate Acquisition.Life (Basel, Switzerland) · 2023Review
- Retinoic Acid Receptor β Controls Development of Striatonigral Projection Neurons through FGF-Dependent and Meis1-Dependent Mechanisms.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- DNA methylation restricts lineage-specific functions of transcription factor Gata4 during embryonic stem cell differentiation.PLoS genetics · 2013Article
- Extra-embryonic endoderm cells derived from ES cells induced by GATA factors acquire the character of XEN cells.BMC developmental biology · 2007Article
- GATA-4 interacts distinctively with negative and positive regulatory elements in the Fgf-3 promoter.Nucleic acids research · 2002Article
- SOX6 binds CtBP2 to repress transcription from the Fgf-3 promoter.Nucleic acids research · 2001Article
- Molecular determinants of the estrogen receptor-coactivator interface.Molecular and cellular biology · 1999Article
- Expression of Fgf-3 in relation to hindbrain segmentation, otic pit position and pharyngeal arch morphology in normal and retinoic acid-exposed mouse embryos.Anatomy and embryology · 1996Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The proto-oncogene Fgf-3 has been implicated as an important signalling molecule in vertebrate development. In the mouse, it is expressed for a limited time at a multitude of sites from embryonic day 7 to birth. Transcription of Fgf-3 initiates at three promoter regions resulting in the generation of various mRNAs which nevertheless all encode the same protein products. A 1.7kb DNA fragment which encompasses these regions was joined to the CAT reporter gene and shown to function as a promoter in embryonal carcinoma cells. In stable transfectants the promoter retains its retinoic acid inducibility, initiating transcription at the same cap-sites as the endogenous gene. In differentiated F9 cells, transient transfection of progressive and targeted deletion mutants of the promoter region has revealed at least two positive and three negative regulatory elements. With one exception, loss of these elements was shown to dramatically affect promoter activity in stable transfectants of F9 cells. However the promoter remained inducible by retinoic acid to differing degrees, apart from deletions encompassing PS-4A which essentially abolished promoter activity in both undifferentiated and differentiated cells. The sequences of these potential regulatory regions were further defined using DNase-I footprinting, revealing some similarities to consensus binding sites for known transcription factors.
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Registered trials
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