ArticleNucleic acids research1993
Enzymic removal of 5-methylcytosine from DNA by a human DNA-glycosylase.
Article in Nucleic acids research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- An enzyme-coupled microplate assay for activity and inhibition of hmdUMP hydrolysis by DNPH1.Analytical biochemistry · 2023Article
- Recent Advances on DNA Base Flipping: A General Mechanism for Writing, Reading, and Erasing DNA Modifications.Advances in experimental medicine and biology · 2022Article
- Urine 5MedC, a Marker of DNA Methylation, in the Progression of Chronic Kidney Disease.Disease markers · 2019Article
- DNA methylation dynamics of genomic imprinting in mouse development.Biology of reproduction · 2018Review
- DNA Base Flipping: A General Mechanism for Writing, Reading, and Erasing DNA Modifications.Advances in experimental medicine and biology · 2016Review
- Measurement of Postreplicative DNA Metabolism and Damage in the Rodent Brain.Chemical research in toxicology · 2015Article
- The Mechanisms of Generation, Recognition, and Erasure of DNA 5-Methylcytosine and Thymine Oxidations.The Journal of biological chemistry · 2015Review
- The carboxy-terminal domain of ROS1 is essential for 5-methylcytosine DNA glycosylase activity.Journal of molecular biology · 2014Article
- Insights into the epigenetic mechanisms controlling pancreatic carcinogenesis.Cancer letters · 2013Review
- Epigenetic regulation in pluripotent stem cells: a key to breaking the epigenetic barrier.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- DNA methylation: dynamic and stable regulation of memory.Biomolecular concepts · 2011Article
- Active DNA demethylation mediated by DNA glycosylases.Annual review of genetics · 2009Review
- Acetylation-induced transcription is required for active DNA demethylation in methylation-silenced genes.Molecular and cellular biology · 2007Article
- Role of the Arabidopsis DNA glycosylase/lyase ROS1 in active DNA demethylation.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- T:G mismatch-specific thymine-DNA glycosylase (TDG) as a coregulator of transcription interacts with SRC1 family members through a novel tyrosine repeat motif.Nucleic acids research · 2005Article
- Overexpression of 5-methylcytosine DNA glycosylase in human embryonic kidney cells EcR293 demethylates the promoter of a hormone-regulated reporter gene.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- An unexpectedly high excision capacity for mispaired 5-hydroxymethyluracil in human cell extracts.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- 5-methylcytosine-DNA glycosylase activity is present in a cloned G/T mismatch DNA glycosylase associated with the chicken embryo DNA demethylation complex.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Modulation of DNA binding protein affinity directly affects target site demethylation.Molecular and cellular biology · 2000Article
- DNA methylation is a reversible biological signal.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
DNA 5-methylcytosine is a major factor in the silencing of mammalian genes; it is involved in gene expression, differentiation, embryogenesis and neoplastic transformation. A decrease in DNA 5-methylcytosine content is associated with activation of specific genes. There is much evidence indicating this to be an enzymic process, with replacement of 5-methylcytosine by cytosine. We demonstrate here enzymic release of 5-methylcytosines from DNA by a human 5-methylcytosine-DNA glycosylase activity, which affords a possible mechanism for such replacement. This activity generates promutagenic apyrimidinic sites, which can be related to the high frequency of mutations found at DNA 5-methylcytosine loci. The recovery of most released pyrimidines as thymines indicates subsequent deamination of free 5-methylcytosines by a 5-methylcytosine deaminase activity. This prevents possible recycling of 5-methylcytosine into replicative DNA synthesis via a possible 5-methyl-dCTP intermediate synthesized through the pyrimidine salvage pathway. Taken together, these findings indicate mechanisms for removal of 5-methylcytosines from DNA, hypermutability of DNA 5-methylcytosine sites, and exclusion of 5-methylcytosines from DNA during replication.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.