ArticleMolecular and cellular biology1994
TATA-binding protein and nuclear differentiation in Tetrahymena thermophila.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Phosphoglycerate kinase: structural aspects and functions, with special emphasis on the enzyme from Kinetoplastea.Open biology · 2020Review
- A germline-limited piggyBac transposase gene is required for precise excision in Tetrahymena genome rearrangement.Nucleic acids research · 2017Article
- Dissecting relative contributions of cis- and trans-determinants to nucleosome distribution by comparing Tetrahymena macronuclear and micronuclear chromatin.Nucleic acids research · 2016Article
- Study of an RNA helicase implicates small RNA-noncoding RNA interactions in programmed DNA elimination in Tetrahymena.Genes & development · 2008Article
- Deposition and function of histone H3 variants in Tetrahymena thermophila.Molecular and cellular biology · 2006Article
- Molecular genetic analysis of an SNF2/brahma-related gene in Tetrahymena thermophila suggests roles in growth and nuclear development.Eukaryotic cell · 2006Article
- A Dicer-like protein in Tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase.Genes & development · 2005Article
- RNA polymerase II localizes in Tetrahymena thermophila meiotic micronuclei when micronuclear transcription associated with genome rearrangement occurs.Eukaryotic cell · 2004Article
- DNA replication and transcription in new macronuclei of Paramecium caudatum exconjugants.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2003Article
- Identification and characterization of the RAD51 gene from the ciliate Tetrahymena thermophila.Nucleic acids research · 1998Article
- A promoter region mutation affecting replication of the Tetrahymena ribosomal DNA minichromosome.Molecular and cellular biology · 1998Article
- An unusual fibrillarin gene and protein: structure and functional implications.Molecular biology of the cell · 1997Article
- Phosphorylation of linker histone is associated with transcriptional activation in a normally silent nucleus.The Journal of cell biology · 1996Article
- A new class of activation-defective TATA-binding protein mutants: evidence for two steps of transcriptional activation in vivo.Molecular and cellular biology · 1996Article
- Phosphorylated and dephosphorylated linker histone H1 reside in distinct chromatin domains in Tetrahymena macronuclei.Molecular biology of the cell · 1995Article
- Developmentally regulated, low abundance Tec element transcripts in Euplotes crassus--implications for DNA elimination and transposition.Nucleic acids research · 1994Article
- The DNA of ciliated protozoa.Microbiological reviews · 1994Review
- DNA rearrangements directed by non-coding RNAs in ciliates.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Unambiguous TATA boxes have not been identified in upstream sequences of Tetrahymena thermophila genes analyzed to date. To begin a characterization of the promoter requirements for RNA polymerase II, the gene encoding TATA-binding protein (TBP) was cloned from this species. The derived amino acid sequence for the conserved C-terminal domain of Tetrahymena TBP is one of the most divergent described and includes a unique 20-amino-acid C-terminal extension. Polyclonal antibodies generated against a fragment of Tetrahymena TBP recognize a 36-kDa protein in macronuclear preparations and also cross-react with yeast and human TBPs. Immunocytochemistry was used to examine the nuclear localization of TBP during growth, starvation, and conjugation (the sexual phase of the life cycle). The transcriptionally active macronuclei stained at all stages of the life cycle. The transcriptionally inert micronuclei did not stain during growth or starvation but surprisingly stained with anti-TBP throughout early stages of conjugation. Anti-TBP staining disappeared from developing micronuclei late in conjugation, corresponding to the onset of transcription in developing macronuclei. Since micronuclei do not enlarge or divide at this time, loss of TBP appears to be an active process. Thus, the transcriptional differences between macro- and micronuclei that arise during conjugation are associated with the loss of a major component of the basal transcription apparatus from developing micronuclei rather than its appearance in developing macronuclei.
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