ArticleMolecular and cellular biology1994
A library of yeast genomic MCM1 binding sites contains genes involved in cell cycle control, cell wall and membrane structure, and metabolism.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 74 citations in OpenAlex.
- Binding and Regulation of Transcription by Yeast Ste12 Variants To Drive Mating and Invasion Phenotypes.Genetics · 2020Article
- Yeast Cip1 is activated by environmental stress to inhibit Cdk1-G1 cyclins via Mcm1 and Msn2/4.Nature communications · 2017Article
- Acetyl-CoA induces transcription of the key G1 cyclin CLN3 to promote entry into the cell division cycle in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The cell wall sensors Mtl1, Wsc1, and Mid2 are required for stress-induced nuclear to cytoplasmic translocation of cyclin C and programmed cell death in yeast.Oxidative medicine and cellular longevity · 2013Article
- A genomewide screen for tolerance to cationic drugs reveals genes important for potassium homeostasis in Saccharomyces cerevisiae.Eukaryotic cell · 2011Article
- An integrated framework to model cellular phenotype as a component of biochemical networks.Advances in bioinformatics · 2011Article
- Coupling phosphate homeostasis to cell cycle-specific transcription: mitotic activation of Saccharomyces cerevisiae PHO5 by Mcm1 and Forkhead proteins.Molecular and cellular biology · 2009Article
- Role of Plc1p in regulation of Mcm1p-dependent genes.FEMS microbiology letters · 2009Article
- Microarray profiling of phage-display selections for rapid mapping of transcription factor-DNA interactions.PLoS genetics · 2009Article
- Use of PMA1 as a housekeeping biomarker for assessment of toxicant-induced stress in Saccharomyces cerevisiae.Applied and environmental microbiology · 2006Article
- N-terminal arm of Mcm1 is required for transcription of a subset of genes involved in maintenance of the cell wall.Eukaryotic cell · 2005Article
- Genomic analysis of PIS1 gene expression.Eukaryotic cell · 2005Article
- The essential transcription factor Reb1p interacts with the CLB2 UAS outside of the G2/M control region.Nucleic acids research · 2003Article
- Factors controlling cyclin B expression.Plant molecular biology · 2000Review
- NDD1, a high-dosage suppressor of cdc28-1N, is essential for expression of a subset of late-S-phase-specific genes in Saccharomyces cerevisiae.Molecular and cellular biology · 1999Article
- Article
- Intracellular glycerol levels modulate the activity of Sln1p, a Saccharomyces cerevisiae two-component regulator.The Journal of biological chemistry · 1999Article
- A MADS box protein consensus binding site is necessary and sufficient for activation of the opaque-phase-specific gene OP4 of Candida albicans.Journal of bacteriology · 1998Article
- Regulation of Cdc28 cyclin-dependent protein kinase activity during the cell cycle of the yeast Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 1998Review
- MAP kinase pathways in the yeast Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 1998Review
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Saccharomyces cerevisiae MCM1 protein, which is essential for viability, participates in both transcription activation and repression as well as DNA replication. However, neither the full network of genes at which MCM1 acts nor whether MCM1 itself mediates a regulatory response is known. Thus far, sites of MCM1 action have been identified by chance during analysis of particular genes. To identify a more complete set of genes on which MCM1 acts, we isolated a library of yeast genomic sequences to which MCM1 binds and then identified known genes within this library. Fragments of genomic DNA, bound to bacterially expressed MCM1 protein, were collected on a nitrocellulose filter, cloned, and analyzed. This selected library contains a large number of genes. As expected, it is enriched for strong MCM1 binding sites and contains cell-type-specific genes known to require MCM1. In addition, it also includes sequences upstream (or near the 5' end) of a number of identified yeast genes that have not yet been shown to be controlled by MCM1. These include genes whose products are involved in (i) the control of cell cycle progression (CLN3, CLB2, and FAR1), (ii) synthesis and maintenance of cell wall or cell membrane structures (PMA1, PIS1, DIT1,2, and GFA1), (iii) cellular metabolism (PCK1, MET2, and CCP1), and (iv) production of a secreted glycoprotein which is heat shock inducible (HSP150). The previously unidentified MCM1 binding site in the essential PMA1 gene is required for expression of a PMA1:lacZ fusion gene, providing evidence that one site is functionally important. We speculate that MCM1 coordinates decisions about cell cycle progression with changes in cell wall integrity and metabolic activity. The presence in the library of three genes involved in cell cycle progression reinforces the idea that one of the functions of MCM1 is indeed analogous to that of the mammalian serum response factor.
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