ArticleMolecular & general genetics : MGG1993
Characterisation of PDC2, a gene necessary for high level expression of pyruvate decarboxylase structural genes in Saccharomyces cerevisiae.
Article in Molecular & general genetics : MGG, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 70 citations in OpenAlex.
- Regulation of thiamine and pyruvate decarboxylase genes by Pdc2 in Nakaseomyces glabratus (Candida glabrata) is complex.G3 (Bethesda, Md.) · 2024Article
- Spontaneous Attenuation of Alcoholic Fermentation via the Dysfunction of Cyc8p inInternational journal of molecular sciences · 2023Article
- Pyruvate decarboxylase and thiamine biosynthetic genes are regulated differently by Pdc2 in S. cerevisiae and C. glabrata.PloS one · 2023Article
- Vitamin requirements and biosynthesis in Saccharomyces cerevisiae.Yeast (Chichester, England) · 2020Review
- Co-opting the fermentation pathway for tombusvirus replication: Compartmentalization of cellular metabolic pathways for rapid ATP generation.PLoS pathogens · 2019Article
- Construction of low-ethanol-wine yeasts through partial deletion of the Saccharomyces cerevisiae PDC2 gene.AMB Express · 2017Article
- The switch from fermentation to respiration in Saccharomyces cerevisiae is regulated by the Ert1 transcriptional activator/repressor.Genetics · 2014Article
- Substrate specificity of thiamine pyrophosphate-dependent 2-oxo-acid decarboxylases in Saccharomyces cerevisiae.Applied and environmental microbiology · 2012Article
- Anaplerotic role for cytosolic malic enzyme in engineered Saccharomyces cerevisiae strains.Applied and environmental microbiology · 2011Article
- The Ehrlich pathway for fusel alcohol production: a century of research on Saccharomyces cerevisiae metabolism.Applied and environmental microbiology · 2008Review
- Pdc2 coordinates expression of the THI regulon in the yeast Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2006Article
- SYM1 is the stress-induced Saccharomyces cerevisiae ortholog of the mammalian kidney disease gene Mpv17 and is required for ethanol metabolism and tolerance during heat shock.Eukaryotic cell · 2004Article
- Pyruvate decarboxylases from the petite-negative yeast Saccharomyces kluyveri.Molecular genetics and genomics : MGG · 2004Article
- Functional redundancies, distinct localizations and interactions among three fission yeast homologs of centromere protein-B.Genetics · 2001Article
- Engineering of the pyruvate dehydrogenase bypass in Saccharomyces cerevisiae: role of the cytosolic Mg(2+) and mitochondrial K(+) acetaldehyde dehydrogenases Ald6p and Ald4p in acetate formation during alcoholic fermentation.Applied and environmental microbiology · 2000Article
- PCNA binding proteins in Drosophila melanogaster : the analysis of a conserved PCNA binding domain.Nucleic acids research · 1998Article
- Cloning and characterization of two pyruvate decarboxylase genes from Pichia stipitis CBS 6054.Applied and environmental microbiology · 1998Article
- Purification and characterization of a CENP-B homologue protein that binds to the centromeric K-type repeat DNA of Schizosaccharomyces pombe.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Article
- A centromere DNA-binding protein from fission yeast affects chromosome segregation and has homology to human CENP-B.The Journal of cell biology · 1997Article
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1 author at 1 institution in 1 country.
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Abstract
The regulatory gene PDC2 was identified in a screen for mutations affecting pyruvate decarboxylase activity in yeast. I have cloned and sequenced this gene. The predicted protein of 925 amino acids has no homology to any sequence in the databases. However, the protein sequence is rich in asparagine and serine residues, as is often found for transcriptional regulators. The PDC2 deletion mutant exhibits a phenotype very similar to, but more severe than that of the point mutant: a strongly reduced pyruvate decarboxylase specific activity, slow, respiration-dependent growth on glucose, and accumulation of pyruvate. The activity of other glycolytic enzymes seems to be unaffected by the pdc2 delta mutation. Synthesis of pyruvate decarboxylase is regulated by PDC2 at the transcriptional level. Expression of the major structural gene for pyruvate decarboxylase, PDC1, is strongly reduced in pdc2 delta mutants. Transcription of the generally more weakly expressed PDC5 gene appears to be entirely abolished. However, glucose induction of pyruvate decarboxylase synthesis is unaffected. Thus, PDC2 is either important for a high basal level of PDC gene expression or it plays a positive role in the autoregulation that controls expression of PDC1 and PDC5.
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