ArticleMolecular & general genetics : MGG1993
Cloning, sequencing and enhanced expression of the Trichoderma reesei endoxylanase II (pI 9) gene xln2.
Article in Molecular & general genetics : MGG, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 70 citations in OpenAlex.
- Influence of codon optimization, promoter, and strain selection on the heterologous production of a β-fructofuranosidase from Aspergillus fijiensis ATCC 20611 in Pichia pastoris.Folia microbiologica · 2022Article
- Rational engineering of xylanase hyper-producing system in Trichoderma reesei for efficient biomass degradation.Biotechnology for biofuels · 2021Article
- Industrial Use of Cell Wall Degrading Enzymes: The Fine Line Between Production Strategy and Economic Feasibility.Frontiers in bioengineering and biotechnology · 2020Review
- Semirational Directed Evolution of Loop Regions in Aspergillus japonicus β-Fructofuranosidase for Improved Fructooligosaccharide Production.Applied and environmental microbiology · 2015Article
- Expression of endo-1, 4-beta-xylanase from Trichoderma reesei in Pichia pastoris and functional characterization of the produced enzyme.BMC biotechnology · 2009Article
- Regulation of xylanase in Aspergillus phoenicis: a physiological and molecular approach.Journal of industrial microbiology & biotechnology · 2008Article
- A new look at xylanases: an overview of purification strategies.Molecular biotechnology · 2003Review
- Transcriptional regulation of xyn2 in Hypocrea jecorina.Eukaryotic cell · 2003Article
- The enzymatic activity of fungal xylanase is not necessary for its elicitor activity.Plant physiology · 1999Article
- Molecular cloning and transcriptional regulation of the Aspergillus nidulans xlnD gene encoding a beta-xylosidase.Applied and environmental microbiology · 1998Article
- The beta-D-xylosidase of Trichoderma reesei is a multifunctional beta-D-xylan xylohydrolase.The Biochemical journal · 1997Article
- Molecular cloning and expression in Saccharomyces cerevisiae of two Aspergillus nidulans xylanase genes.Applied and environmental microbiology · 1996Article
- Expression of a Trichoderma reesei beta-xylanase gene (XYN2) in Saccharomyces cerevisiae.Applied and environmental microbiology · 1996Article
- Production of Trichoderma reesei cellulases on glucose-containing media.Applied and environmental microbiology · 1995Article
- Cloning and expression in Saccharomyces cerevisiae of a Trichoderma reesei beta-mannanase gene containing a cellulose binding domain.Applied and environmental microbiology · 1995Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The Trichoderma reesei xln2 gene coding for the pI9.0 endoxylanase was isolated from the wild-type strain QM6a. The gene contains one intron of 108 nucleotides and codes for a protein of 223 amino acids in which two putative N-glycosylation target sites were found. Three different T. reesei strains were transformed by targeting a construct composed of the xln2 gene, including its promoter, to the endogenous cbh1 locus. Highest overall production levels of xylanase were obtained using T. reesei ALKO2721, a genetically engineered strain, as a host. Integration into the cbh1 locus was not required for enhanced expression under control of the xln2 promoter.
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