ArticleImmunology1993
CD69 is expressed by human eosinophils activated in vivo in asthma and in vitro by cytokines.
Article in Immunology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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Who cites it
26 citing papers in PubMed, 148 citations in OpenAlex.
- Combining bacterial display and protein language models to engineer a CD69-binding affibody for molecular imaging of immune activation.Protein engineering, design & selection : PEDS · 2026Article
- Inhibitory Effects of Phellodendri Cortex Against Airway Inflammation and Hyperresponsiveness in Ovalbumin-Induced Murine Asthma Model.Molecules (Basel, Switzerland) · 2025Article
- Immuno-PET Imaging of CD69 Visualizes T-Cell Activation and Predicts Survival Following Immunotherapy in Murine Glioblastoma.Cancer research communications · 2023Article
- Peripheral blood eosinophils priming andPostepy dermatologii i alergologii · 2021Article
- Discovery, optimization and biodistribution of an Affibody molecule for imaging of CD69.Scientific reports · 2021Article
- Interleukin 7 receptor is required for myeloid cell homeostasis and reconstitution by hematopoietic stem cells.Experimental hematology · 2020Article
- Eosinophil accumulation predicts response to melanoma treatment with immune checkpoint inhibitors.Oncoimmunology · 2020Article
- Mepolizumab Attenuates Airway Eosinophil Numbers, but Not Their Functional Phenotype, in Asthma.American journal of respiratory and critical care medicine · 2017Article
- Microparticles in nasal lavage fluids in chronic rhinosinusitis: Potential biomarkers for diagnosis of aspirin-exacerbated respiratory disease.The Journal of allergy and clinical immunology · 2017Article
- Eosinophil Activation Status in Separate Compartments and Association with Asthma.Frontiers in medicine · 2017Review
- Essential Mechanisms of Differential Activation of Eosinophils by IL-3 Compared to GM-CSF and IL-5.Critical reviews in immunology · 2016Review
- Activation states of blood eosinophils in asthma.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2014Review
- Eosinophil cytokines, chemokines, and growth factors: emerging roles in immunity.Frontiers in immunology · 2014Review
- Is CD69 an effective brake to control inflammatory diseases?Trends in molecular medicine · 2013Review
- Impaired lymphocyte profile in schistosomiasis patients with periportal fibrosis.Clinical & developmental immunology · 2013Article
- Eosinophils: multifaceted biological properties and roles in health and disease.Immunological reviews · 2011Review
- Potential contribution of IL-7 to allergen-induced eosinophilic airway inflammation in asthma.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Expression of interleukin-13 receptor alpha 1-subunit on peripheral blood eosinophils is regulated by cytokines.Immunology · 2004Article
- Prolonged allergen challenge in mice leads to persistent airway remodelling.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2004Article
- Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects.Clinical and experimental immunology · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CD69 is an early activation marker for T cells and cross-linking of CD69 on platelets triggers aggregation and mediator release. Expression of a number of membrane receptors is induced on eosinophils after culture with certain cytokines. Therefore, we investigated whether cytokine-activated eosinophils expressed CD69. Unstimulated, peripheral blood eosinophils did not express CD69, as determined by immunofluorescence and flow cytometry (n = 15). CD69 expression was induced on eosinophils by granulocyte-macrophage colony-stimulating factor (GM-CSF) in a time- and dose-dependent manner. After 1 day in culture, expression was significant at concentrations of 10(-11) M and above. CD69 expression could be detected after stimulation with GM-CSF for only 1 hr, was significant after 2 hr and was sustained over 1-2 days in culture. CD69 expression was also induced by interleukin-3 (IL-3), IL-5 and interferon-gamma (IFN-gamma), but stimulation of eosinophils with platelet-activating factor (PAF) (10(-6) M) for up to 2 hr did not induce CD69 expression. Cycloheximide (10(-6) M) significantly inhibited GM-CSF-induced CD69 expression, suggesting a requirement for protein synthesis. However, unlike up-regulation of CR3 expression, GM-CSF-induced CD69 expression was not inhibited by dexamethasone. CD69 was present on eosinophils from the bronchoalveolar lavage (BAL) fluid of patients with mild asthma (5/5), suggesting that the in vitro findings may have biological relevance in vivo. Therefore, CD69 can be used as a marker of eosinophil activation by cytokines and is a candidate receptor for triggering eosinophil mediator release in the airways in asthma.
Indexed as
Identifiers
8262555PMC1422202W50347659What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.