ArticleThe Biochemical journal1993
Activation of NADPH oxidase of human neutrophils involves the phosphorylation and the translocation of cytosolic p67phox.
Article in The Biochemical journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 97 citations in OpenAlex.
- NADPH Oxidase 3: Beyond the Inner Ear.Antioxidants (Basel, Switzerland) · 2024Review
- Article
- Phosphorylation of Nox1 regulates association with NoxA1 activation domain.Circulation research · 2014Article
- Assembly of NADPH oxidase in human neutrophils is modulated by the opacity-associated protein expression State of Neisseria gonorrhoeae.Infection and immunity · 2014Article
- Elevated NADPH oxidase activity contributes to oxidative stress and cell death in Huntington's disease.Human molecular genetics · 2013Article
- Biochemistry, physiology, and pathophysiology of NADPH oxidases in the cardiovascular system.Circulation research · 2012Review
- Characterization of superoxide overproduction by the D-Loop(Nox4)-Nox2 cytochrome b(558) in phagocytes-Differential sensitivity to calcium and phosphorylation events.Biochimica et biophysica acta · 2011Article
- Emerging evidence for the importance of phosphorylation in the regulation of NADPH oxidases.Antioxidants & redox signaling · 2009Review
- Platelet-activating factor-mediated endosome formation causes membrane translocation of p67phox and p40phox that requires recruitment and activation of p38 MAPK, Rab5a, and phosphatidylinositol 3-kinase in human neutrophils.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- A specific p47phox -serine phosphorylated by convergent MAPKs mediates neutrophil NADPH oxidase priming at inflammatory sites.The Journal of clinical investigation · 2006Article
- Activation and assembly of the NADPH oxidase: a structural perspective.The Biochemical journal · 2005Review
- Assembly of the phagocyte NADPH oxidase.Histochemistry and cell biology · 2004Review
- The NADPH oxidase of professional phagocytes--prototype of the NOX electron transport chain systems.Biochimica et biophysica acta · 2004Review
- Superoxide generation of phagocytes and nonphagocytic cells.Protoplasma · 2001Review
- Chronic granulomatous disease.Clinical and experimental immunology · 2000Review
- Intracellular reactions in single human granulocytes upon phorbol myristate acetate activation using confocal Raman microspectroscopy.Biophysical journal · 2000Article
- Direct interaction between p47phox and protein kinase C: evidence for targeting of protein kinase C by p47phox in neutrophils.The Biochemical journal · 1999Article
- Detection of oxidant producing-sites in glutaraldehyde-fixed human neutrophils and eosinophils stimulated with phorbol myristate acetate.The Histochemical journal · 1999Article
- The major phosphorylation site of the NADPH oxidase component p67phox is Thr233.The Biochemical journal · 1999Article
- Modulation of neutrophil superoxide response and intracellular diacylglyceride levels by the bacterial pigment pyocyanin.Infection and immunity · 1997Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Activation of human neutrophil NADPH oxidase requires the interaction of cytosolic and membrane-associated components. Evidence has been accumulated that in phorbol 12-myristate 13-acetate (PMA)-stimulated neutrophils, the translocation to the plasma membrane of the cytosolic components p47phox and p67phox and the phosphorylation of p47phox are essential steps in activation of NADPH oxidase. No direct evidence has been presented to date as to whether p67phox is also phosphorylated. To address this problem we have immunoprecipitated p67phox from neutrophil cytosol and membrane fractions. The results indicate that, very soon after activation with PMA (20 s), p67phox was present in a phosphorylated form in the cytosol and in the membranes. At later times (1-3 min) the extent of p67phox phosphorylation continuously increased both in the cytosol and in the membrane fraction, while oxygen consumption reached the maximal rate within 40 s, and then remained linear. p67phox was also phosphorylated in formyl-methionyl-leucyl-phenylalanine-activated neutrophils. That the phosphorylated p67 protein we identified in immunoprecipitation experiments was p67phox was confirmed by the observation that no phosphorylated band of 67 kDa was immunoprecipitated from the cytosol and membranes of PMA-stimulated neutrophils from a p67phox-deficient chronic granulomatous disease patient. In this case, p47phox was normally phosphorylated. These data demonstrate that: (1) the phosphorylation of p67phox is correlated with activation of NADPH oxidase, and (2) continuous phosphorylation of p67phox is required in order to maintain the linearity of the respiratory burst.
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