ArticleNucleic acids research1993
Polyadenylation site selection cannot occur in vivo after excision of the 3'-terminal intron.
Article in Nucleic acids research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- A simple model to explain evolutionary trends of eukaryotic gene architecture and expression: how competition between splicing and cleavage/polyadenylation factors may affect gene expression and splice-site recognition in eukaryotes.BioEssays : news and reviews in molecular, cellular and developmental biology · 2013Review
- Binding of hnRNP L to the pre-mRNA processing enhancer of the herpes simplex virus thymidine kinase gene enhances both polyadenylation and nucleocytoplasmic export of intronless mRNAs.Molecular and cellular biology · 2005Article
- Novel alternative splicing predicts a truncated isoform of the NMDA receptor subunit 1 (NR1) in embryonic rat brain.Neurochemical research · 2005Article
- Pre-mRNA processing enhancer (PPE) elements from intronless genes play additional roles in mRNA biogenesis than do ones from intron-containing genes.Nucleic acids research · 2005Article
- Use of intron-disrupted polyadenylation sites to enhance expression and safety of retroviral vectors.Journal of virology · 2001Article
- Intronless mRNA transport elements may affect multiple steps of pre-mRNA processing.The EMBO journal · 1999Article
- Transcriptional termination in the Balbiani ring 1 gene is closely coupled to 3'-end formation and excision of the 3'-terminal intron.Genes & development · 1998Article
- The mouse histone H2a gene contains a small element that facilitates cytoplasmic accumulation of intronless gene transcripts and of unspliced HIV-1-related mRNAs.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- The HIV-1 5' LTR poly(A) site is inactivated by U1 snRNP interaction with the downstream major splice donor site.The EMBO journal · 1997Article
- Herpes simplex virus type 1 protein IE63 affects the nuclear export of virus intron-containing transcripts.Journal of virology · 1996Article
- Sequence of the polypyrimidine tract of the 3'-terminal 3' splicing signal can affect intron-dependent pre-mRNA processing in vivo.Nucleic acids research · 1996Article
- Trans-splicing and alternative-tandem-cis-splicing: two ways by which mammalian cells generate a truncated SV40 T-antigen.Nucleic acids research · 1996Article
- Expression of the Volvox gene encoding nitrate reductase: mutation-dependent activation of cryptic splice sites and intron-enhanced gene expression from a cDNA.Plant molecular biology · 1996Article
- Regulation of herpes simplex virus poly (A) site usage and the action of immediate-early protein IE63 in the early-late switch.Journal of virology · 1996Article
- Generation of a high-titer retroviral vector capable of expressing high levels of the human beta-globin gene.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Lack of an effect of the efficiency of RNA 3'-end formation on the efficiency of removal of either the final or the penultimate intron in intact cells.Molecular and cellular biology · 1995Article
- A genetic polymorphism within the third poly(A) signal of the DHFR gene alters the polyadenylation pattern of DHFR transcripts in CHL cells.Nucleic acids research · 1994Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Splicing of 3'-terminal introns and polyadenylation of pre-mRNAs can be coupled in an appropriate cell-free system. However, definitive evidence has been lacking as to whether these events are coupled in vivo and whether the order of these two processing events is obligatory. Here, we investigated these questions by examining the in vivo processing of transcripts that differ solely by the precise insertion of an intron within the first of two polyadenylation signals. Quantitative S1 nuclease mapping and PCR techniques were utilized to analyze the processed RNAs that accumulated in monkey cells transfected with plasmids encoding these transcripts. We found that, whereas all of the primary transcripts that lacked the inserted intron were processed via utilization of the 5'-proximal polyadenylation signal, none of the transcripts initially disrupted in this signal were processed this way even though the disrupting intron had been properly excised and excision sometimes preceded polyadenylation. In addition, deletion of the second polyadenylation signal resulted in failure of spliced transcripts to accumulate. We conclude that selection of, but not necessarily cleavage at the polyadenylation site precedes excision of the 3'-terminal intron in vivo; although coupling exists during selection of the sites to be used for polyadenylation and excision of the 3'-terminal intron, the actual order of the subsequent enzymatic reactions is probably simply a reflection of their relative kinetics.
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