ArticleNucleic acids research1993
Reconstitution of mammalian excision repair activity with mutant cell-free extracts and XPAC and ERCC1 proteins expressed in Escherichia coli.
Article in Nucleic acids research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 18 citations in OpenAlex.
- Photo-cross-linking of XPC-Rad23B to cisplatin-damaged DNA reveals contacts with both strands of the DNA duplex and spans the DNA adduct.Biochemistry · 2010Article
- Assembly, subunit composition, and footprint of human DNA repair excision nuclease.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Specific association between the human DNA repair proteins XPA and ERCC1.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Formation of a ternary complex by human XPA, ERCC1, and ERCC4(XPF) excision repair proteins.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Nucleotide excision repair in humans involves the coordinated actions of 8-10 proteins. To understand the roles of each of these proteins in excision it is necessary to develop an in vitro excision repair system reconstituted entirely from purified proteins. Towards this goal we have expressed in E. coli two of the 8 genes known to be essential for the excision reaction. XPAC and ERCC1 were expressed as fusion proteins with the Escherichia coli maltose binding protein (MBP) and purified to > 80% homogeneity by affinity chromatography. The purified proteins either as fusions or after cleavage from the MBP were able to complement the CFE of cells with mutations in the corresponding genes in an excision assay with thymine dimer containing substrate.
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