ArticleNucleic acids research1993
The Drosophila copia retrotransposon contains binding sites for transcriptional regulation by homeoproteins.
Article in Nucleic acids research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 31 citations in OpenAlex.
- Copia retrotransposon in the Zaprionus genus: another case of transposable element sharing with the Drosophila melanogaster subgroup.Journal of molecular evolution · 2011Article
- Multiple invasions of Gypsy and Micropia retroelements in genus Zaprionus and melanogaster subgroup of the genus Drosophila.BMC evolutionary biology · 2009Article
- Quantitative genetic analysis of copia retrotransposon activity in inbred Drosophila melanogaster lines.Genetics · 1998Article
- Steroid hormone responsiveness of a family of closely related mouse proviral elements.Mammalian genome : official journal of the International Mammalian Genome Society · 1997Article
- Molecular cloning and characterization of a transcription factor for the copia retrotransposon with homology to the BTB-containing lola neurogenic factor.Molecular and cellular biology · 1997Article
- Naturally occurring variation in copia expression is due to both element (cis) and host (trans) regulatory variation.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
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2 authors at 1 institution in 1 country.
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Abstract
We have identified in the 5' untranslated region of the Drosophila copia retrotransposon, 3' to the left LTR, a sequence for transcriptional regulation by homeoproteins. Co-transfection assays using expression vectors for homeoproteins and reporter vectors containing the lacZ gene under the control of either the entire copia LTR with 5' untranslated sequence, or a minimal heterologous promoter flanked with a 130 bp fragment containing the copia untranslated region, disclosed both positive and negative modulations of promoter activity in Drosophila cells in culture: a 5-10 fold decrease with engrailed, even-skipped and zerknüllt in DH33 cells, and a 10-30 fold increase with fushi tarazu and zerknüllt in Schneider II cells. In all cases, the regulatory effects were abolished with reporter plasmids deleted for a 58 bp fragment encompassing the putative homeoprotein binding sites. Mobility shift assays with a purified homeodomain-containing peptide demonstrated direct interaction with the 58 bp fragment, with an affinity in the 1-10 nM range as reported with the same peptide for other well characterized homeodomain binding regulatory sites. Foot-printing experiments with the extended LTR demonstrated protection of 'consensus' sequences, located within the 58 bp fragment. These homeodomain binding sites could be involved in the developmental regulation of the copia retrotransposon.
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