ArticleThe Journal of cell biology1993
Neural regulation of muscle acetylcholine receptor epsilon- and alpha-subunit gene promoters in transgenic mice.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 83 citations in OpenAlex.
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- Myonuclear content regulates cell size with similar scaling properties in mice and humans.Nature communications · 2020Article
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- Neuromuscular junctions are pathological but not denervated in two mouse models of spinal bulbar muscular atrophy.Human molecular genetics · 2016Article
- Creating Interactions between Tissue-Engineered Skeletal Muscle and the Peripheral Nervous System.Cells, tissues, organs · 2016Article
- Muscle-derived extracellular signal-regulated kinases 1 and 2 are required for the maintenance of adult myofibers and their neuromuscular junctions.Molecular and cellular biology · 2015Article
- Excitation-transcription coupling in skeletal muscle: the molecular pathways of exercise.Biological reviews of the Cambridge Philosophical Society · 2011Review
- Chromatin modifications that support acetylcholine receptor gene activation are established during muscle cell determination and differentiation.Molecular biology reports · 2011Article
- Myonuclei acquired by overload exercise precede hypertrophy and are not lost on detraining.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Bench-to-bedside review: ventilatory abnormalities in sepsis.Critical care (London, England) · 2009Review
- Nuclear domains during muscle atrophy: nuclei lost or paradigm lost?The Journal of physiology · 2008Review
- MyoD- and nerve-dependent maintenance of MyoD expression in mature muscle fibres acts through the DRR/PRR element.BMC developmental biology · 2008Article
- Neuromuscular synapse formation in mice lacking motor neuron- and skeletal muscle-derived Neuregulin-1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Number and spatial distribution of nuclei in the muscle fibres of normal mice studied in vivo.The Journal of physiology · 2003Article
- Essential roles of c-JUN and c-JUN N-terminal kinase (JNK) in neuregulin-increased expression of the acetylcholine receptor epsilon-subunit.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- An intronic enhancer containing an N-box motif is required for synapse- and tissue-specific expression of the acetylcholinesterase gene in skeletal muscle fibers.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Synapse-specific and neuregulin-induced transcription require an ets site that binds GABPalpha/GABPbeta.Genes & development · 1998Article
- Epsilon subunit-containing acetylcholine receptors in myotubes belong to the slowly degrading population.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Accumulation in fetal muscle and localization to the neuromuscular junction of cAMP-dependent protein kinase A regulatory and catalytic subunits RI alpha and C alpha.The Journal of cell biology · 1996Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effects of denervation were investigated in mice with transgenes containing promoter elements from the muscle acetylcholine receptor epsilon- and alpha-subunit genes. The promoter sequences were coupled to a nuclear localization signal-beta-galactosidase fusion gene (nlacZ) as a reporter. While many postsynaptic specializations form in the embryo, expression of the epsilon subunit is induced during the first two postnatal weeks. When muscles were denervated at birth, before the onset of epsilon expression, epsilon nlacZ still appeared at the former synaptic sites on schedule. This result suggests that the nerve leaves a localized "trace" in the muscle that can continue to regulate transcription. An additional finding was that epsilon nlacZ expression was much stronger in denervated than in intact muscles. This suggests that the epsilon promoter is similar to the other subunits in containing elements that are activated on cessation of neural activity. However, even after denervation, epsilon nlacZ expression was always confined to the synaptic region whereas alpha nlacZ expression increased in nuclei along the entire length of the fiber. This suggests that while the epsilon gene is similar in its activity dependence to other subunit genes, it is unique in that local nerve-derived signals are essential for its expression. Consequently, inactivity enhances epsilon expression only in synaptic nuclei where such signals are present, but enhances expression throughout the muscle fiber. Truncations and an internal deletion of the epsilon promoter indicate that cis-elements essential for the response to synaptic signals are contained within 280 bp of the transcription start site. In contrast to these results in young animals, denervation in older animals leads to an unexpected reduction in nlacZ activity. However, mRNA measurements indicated that transgene expression was increased in these animals. This discordance between nlacZ mRNA and enzyme activity, demonstrates a previously unknown limitation of nlacZ as a reporter gene in transgenic animals.
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