ArticleThe Journal of cell biology1993
The rod domain of NF-L determines neurofilament architecture, whereas the end domains specify filament assembly and network formation.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Deletions in Glial Fibrillary Acidic Protein Leading to Alterations in Intermediate Filament Assembly and Network Formation.International journal of molecular sciences · 2025Article
- Customized antisense oligonucleotide-based therapy for neurofilament-associated Charcot-Marie-Tooth disease.Brain : a journal of neurology · 2024Article
- Neurofilament Biophysics: From Structure to Biomechanics.Molecular biology of the cell · 2024Review
- Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Regulation of neurofilament length and transport by a dynamic cycle of phospho-dependent polymer severing and annealing.Molecular biology of the cell · 2023Article
- Article
- A review and analysis of the clinical literature on Charcot-Marie-Tooth disease caused by mutations in neurofilament protein L.Cytoskeleton (Hoboken, N.J.) · 2021Review
- Cytoskeleton as a Target of Quinolinic Acid Neurotoxicity: Insight from Animal Models.Molecular neurobiology · 2018Review
- Interaction of small heat shock proteins with light component of neurofilaments (NFL).Cell stress & chaperones · 2017Article
- Genetic and clinical characteristics ofJournal of neurology, neurosurgery, and psychiatry · 2017Article
- Serum neurofilament light protein predicts clinical outcome in traumatic brain injury.Scientific reports · 2016Article
- An Atypical Tropomyosin in Drosophila with Intermediate Filament-like Properties.Cell reports · 2016Article
- The tail domain is essential but the head domain dispensable for C. elegans intermediate filament IFA-2 function.PloS one · 2015Article
- Conformational properties of interacting neurofilaments: Monte Carlo simulations of cylindrically grafted apposing neurofilament brushes.Journal of biological physics · 2013Article
- Neurofilament proteins in axonal regeneration and neurodegenerative diseases.Neural regeneration research · 2012Review
- Modeling the self-organization property of keratin intermediate filaments.Biophysical journal · 2010Article
- MSC p43 required for axonal development in motor neurons.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Brain antigens in functionally distinct antigen-presenting cell populations in cervical lymph nodes in MS and EAE.Journal of molecular medicine (Berlin, Germany) · 2009Article
- Cofilin increases the bending flexibility of actin filaments: implications for severing and cell mechanics.Journal of molecular biology · 2008Article
- Review of the multiple aspects of neurofilament functions, and their possible contribution to neurodegeneration.Molecular neurobiology · 2008Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neurofilaments, assembled from NF-L, NF-M, and NF-H subunits, are the most abundant structural elements in myelinated axons. Although all three subunits contain a central, alpha-helical rod domain thought to mediate filament assembly, only NF-L self-assembles into 10-nm filaments in vitro. To explore the roles of the central rod, the NH2-terminal head and the COOH-terminal tail domain in filament assembly, full-length, headless, tailless, and rod only fragments of mouse NF-L were expressed in bacteria, purified, and their structure and assembly properties examined by conventional and scanning transmission electron microscopy (TEM and STEM). These experiments revealed that in vitro assembly of NF-L into bona fide 10-nm filaments requires both end domains: whereas the NH2-terminal head domain promotes lateral association of protofilaments into protofibrils and ultimately 10-nm filaments, the COOH-terminal tail domain controls lateral assembly of protofilaments so that it terminates at the 10-nm filament level. Hence, the two end domains of NF-L have antagonistic effects on the lateral association of protofilaments into higher-order structures, with the effect of the COOH-terminal tail domain being dominant over that of the NH2-terminal head domain. Consideration of the 21-nm axial beading commonly observed with 10-nm filaments, the approximate 21-nm axial periodicity measured on paracrystals, and recent cross-linking data combine to support a molecular model for intermediate filament architecture in which the 44-46-nm long dimer rods overlap by 1-3-nm head-to-tail, whereas laterally they align antiparallel both unstaggered and approximately half-staggered.
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