ArticleThe Journal of cell biology1993
Mechanisms of urinary K+ and H+ excretion: primary structure and functional expression of a novel H,K-ATPase.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 84 citations in OpenAlex.
- Real-time imaging of rotation during synthesis by the replisome.Science advances · 2026Article
- Aldosterone: Renal Action and Physiological Effects.Comprehensive Physiology · 2023Article
- Colonic Potassium Absorption and Secretion in Health and Disease.Comprehensive Physiology · 2018Review
- Proton Pump Inhibitors and Dementia: Physiopathological Mechanisms and Clinical Consequences.Neural plasticity · 2018Review
- Deceleration of the E1P-E2P transition and ion transport by mutation of potentially salt bridge-forming residues Lys-791 and Glu-820 in gastric H+/K+-ATPase.The Journal of biological chemistry · 2010Article
- Pharmacological profiles of the murine gastric and colonic H,K-ATPases.Biochimica et biophysica acta · 2010Article
- Molecular identification of Sch28080-sensitive K-ATPase activities in the mouse kidney.Pflugers Archiv : European journal of physiology · 2006Article
- Does the colonic H,K-ATPase also act as an Na,K-ATPase?Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Electrogenic sulfate/chloride exchange in Xenopus oocytes mediated by murine AE1 E699Q.The Journal of general physiology · 1997Article
- Quantitative RT-PCR analysis of mRNAs encoding a colonic putative H, K-ATPase alpha subunit along the rat nephron: effect of K+ depletion.Pflugers Archiv : European journal of physiology · 1996Article
- Functional expression and segmental localization of rat colonic K-adenosine triphosphatase.The Journal of clinical investigation · 1995Article
- The role of H(+)-ATPases in urinary acidification.Pediatric nephrology (Berlin, Germany) · 1995Review
- Ouabain resistance of the epithelial cell line (Ma104) is not due to lack of affinity of its pumps for the drug.The Journal of membrane biology · 1995Article
- Primary sequence and functional expression of a novel beta subunit of the P-ATPase gene family.Pflugers Archiv : European journal of physiology · 1993Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The kidney plays an essential role in regulating potassium and acid balance. A major site for these regulations is in the collecting tubule. In the present study, we report the primary sequence of a novel alpha subunit of the P-ATPase gene family, which we isolated from the urinary bladder epithelium of the toad Bufo marinus, the amphibian equivalent of the mammalian collecting tubule. The cDNA encodes a protein of 1,042 amino acids which shares approximately 67% identity with the alpha 1 subunit of the ouabain-inhibitable Na,K-ATPase and approximately 69% identity with the alpha subunit of the SCH28080-inhibitable gastric H,K-ATPase. When coexpressed in Xenopus oocytes with a beta subunit isolated from the same cDNA library, the ATPase is able to transport rubidium (a potassium surrogate) inward, and hydrogen outward, leading to alkalization of the intracellular compartment and acidification of the external medium. The novel ATPase has a unique pharmacological profile showing intermediate sensitivity to both ouabain and SCH28080. Our findings indicate that the bladder ATPase is a member of a new ion motive P-ATPase subfamily. The bladder ATPase is expressed in the urinary tract but not in the stomach or the colon. This H,K-ATPase may be one of the molecules involved in H+ and K+ homeostasis, mediating the transport of these ions across urinary epithelia and therefore regulating their urinary excretion.
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