ArticleThe Journal of cell biology1993
Cytoplasmic dynein-dependent vesicular transport from early to late endosomes.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 163 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
163 citing papers in PubMed, 3 syntheses or guidelines pooled it, 406 citations in OpenAlex.
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- Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).Autophagy · 2016Guideline
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- Transcriptomic insights into the shift of trophic strategies in mixotrophic dinoflagellateISME communications · 2024Article
- Small Sized Yet Powerful: Nuclear Distribution C Proteins in Plants.Plants (Basel, Switzerland) · 2023Review
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- Nucleic Acid-Loaded Lipid Nanoparticle Interactions with Model Endosomal Membranes.ACS applied materials & interfaces · 2022Article
- Chasing a Breath of Fresh Air in Cystic Fibrosis (CF): Therapeutic Potential of Selective HDAC6 Inhibitors to Tackle Multiple Pathways in CF Pathophysiology.Journal of medicinal chemistry · 2022Review
- Engineered EV-Mimetic Nanoparticles as Therapeutic Delivery Vehicles for High-Grade Serous Ovarian Cancer.Cancers · 2021Review
- CDK11 negatively regulates Wnt/β-catenin signaling in the endosomal compartment by affecting microtubule stability.Cancer biology & medicine · 2020Article
- PX Domain-Containing Kinesin KIF16B and Microtubule-Dependent Intracellular Movements.The Journal of membrane biology · 2020Review
- Different localization of lysosomal-associated membrane protein 1 (LAMP1) in mammalian cultured cell lines.Histochemistry and cell biology · 2020Article
- Microtubules in Influenza Virus Entry and Egress.Viruses · 2020Review
- Cyclodextrin triggers MCOLN1-dependent endo-lysosome secretion in Niemann-Pick type C cells.Journal of lipid research · 2019Article
- Article
- Downregulation of GABAMolecular neurobiology · 2017Article
- Moesin and cortactin control actin-dependent multivesicular endosome biogenesis.Molecular biology of the cell · 2016Article
- ICAM-1 Binding Rhinoviruses A89 and B14 Uncoat in Different Endosomal Compartments.Journal of virology · 2016Article
103 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have used an in vitro fusion assay to study the mechanisms of transport from early to late endosomes. Our data show that the late endosomes share with the early endosomes a high capacity to undergo homotypic fusion in vitro. However, direct fusion of early with late endosomes does not occur. We have purified vesicles which are intermediates during transport from early to late endosomes in vivo, and analyzed their protein composition in two-dimensional gels. In contrast to either early or late endosomes, these vesicles do not appear to contain unique proteins. Moreover, these vesicles undergo fusion with late endosomes in vitro, but not with each other or back with early endosomes. In vitro, fusion of these endosomal vesicles with late endosomes is stimulated by polymerized microtubules, consistent with the known role of microtubules during early to late endosome transport in vivo. In contrast, homotypic fusion of early or late endosomes is microtubule-independent. Finally, this stimulation by microtubules depends on microtubule-associated proteins and requires the presence of the minus-end directed motor cytoplasmic dynein, but not the plus-end directed motor kinesin, in agreement with the microtubule organization in vivo. Our data strongly suggest that early and late endosomes are separate, highly dynamic organelles, which are connected by a microtubule-dependent vesicular transport step.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.