ArticleThe Journal of cell biology1993
Nup180, a novel nuclear pore complex protein localizing to the cytoplasmic ring and associated fibrils.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 46 citations in OpenAlex.
- Article
- Heterogeneity of autoantibodies in 100 patients with autoimmune myositis: insights into clinical features and outcomes.Arthritis research & therapy · 2007Article
- Autoantigens of the nuclear pore complex.Journal of molecular medicine (Berlin, Germany) · 2004Review
- A role for the divergent actin gene, ACT2, in nuclear pore structure and function.The EMBO journal · 1997Article
- Nucleocytoplasmic transport of macromolecules.Microbiology and molecular biology reviews : MMBR · 1997Review
- Nic96p is required for nuclear pore formation and functionally interacts with a novel nucleoporin, Nup188p.The Journal of cell biology · 1996Article
- Immunolocalization of lamins and nuclear pore complex proteins by atomic force microscopy.Pflugers Archiv : European journal of physiology · 1995Article
- Plant nuclear pore complex proteins are modified by novel oligosaccharides with terminal N-acetylglucosamine.The Plant cell · 1995Article
- Structural interaction between the nuclear pore complex and a specific translocating RNP particle.The Journal of cell biology · 1995Article
- Nuclear pore complex assembly studied with a biochemical assay for annulate lamellae formation.The Journal of cell biology · 1995Article
- Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97, import but are defective in nuclear growth and replication.The Journal of cell biology · 1995Article
- Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p.The EMBO journal · 1995Article
- Tpr, a large coiled coil protein whose amino terminus is involved in activation of oncogenic kinases, is localized to the cytoplasmic surface of the nuclear pore complex.The Journal of cell biology · 1994Article
- Interactions and three-dimensional localization of a group of nuclear pore complex proteins.The Journal of cell biology · 1994Article
- Intranuclear filaments containing a nuclear pore complex protein.The Journal of cell biology · 1993Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using an autoimmune serum from a patient with overlap connective tissue disease we have identified by biochemical and immunocytochemical approaches an evolutionarily conserved nuclear pore complex (NPC) protein with an estimated molecular mass of 180 kD and an isoelectric point of approximately 6.2 which we have designated as nup180. Extraction of isolated nuclear envelopes with 2 M urea and chromatography of the solubilized proteins on WGA-Sepharose demonstrated that nup180 is a peripheral membrane protein and does not react with WGA. Affinity-purified antibodies yielded a punctate immunofluorescent pattern of the nuclear surface of mammalian cells and stained brightly the nuclear envelope of cryosectioned Xenopus oocytes. Nuclei reconstituted in vitro in Xenopus egg extract were also stained in the characteristic punctate fashion. Immunogold EM localized nup180 exclusively to the cytoplasmic ring of NPCs and short fibers emanating therefrom into the cytoplasm. Antibodies to nup180 did not inhibit nuclear protein transport in vivo nor in vitro. Despite the apparent lack of involvement in NPC assembly or nucleocytoplasmic transport processes, the conservation of nup180 across species and its exclusive association with the NPC cytoplasmic ring suggests an important, though currently undefined function for this novel NPC protein.
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