ArticleThe EMBO journal1993
Both Arabidopsis TATA binding protein (TBP) isoforms are functionally identical in RNA polymerase II and III transcription in plant cells: evidence for gene-specific changes in DNA binding specificity of TBP.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- RNA-seq analysis of wild-type and mutated TBPL1 gene in breast cancer cells lines through CRISPR/Cas9 approach reveals novel molecular signatures.Scientific reports · 2025Article
- CRISPRi-based circuits to control gene expression in plants.Nature biotechnology · 2025Article
- The general transcription factors (GTFs) of RNA polymerase II and their roles in plant development and stress responses.Critical reviews in biochemistry and molecular biology · 2024Review
- A critical role of a plant-specific TFIIB-related protein, BRP1, in salicylic acid-mediated immune response.Frontiers in plant science · 2024Article
- On the Role of TATA Boxes and TATA-Binding Protein inPlants (Basel, Switzerland) · 2023Review
- Plant_SNP_TATA_Z-Tester: A Web Service That Unequivocally Estimates the Impact of Proximal Promoter Mutations on Plant Gene Expression.International journal of molecular sciences · 2022Article
- Specific chromatin changes mark lateral organ founder cells in the Arabidopsis inflorescence meristem.Journal of experimental botany · 2019Article
- Diversity of cis-regulatory elements associated with auxin response in Arabidopsis thaliana.Journal of experimental botany · 2018Review
- Genome-wide occupancy profile of the RNA polymerase III machinery in Saccharomyces cerevisiae reveals loci with incomplete transcription complexes.Molecular and cellular biology · 2004Article
- Transcription factor IIB (TFIIB)-related protein (pBrp), a plant-specific member of the TFIIB-related protein family.Molecular and cellular biology · 2003Article
- Mot1 associates with transcriptionally active promoters and inhibits association of NC2 in Saccharomyces cerevisiae.Molecular and cellular biology · 2002Article
- Transcription and tyranny in the nucleolus: the organization, activation, dominance and repression of ribosomal RNA genes.The arabidopsis book · 2002Article
- Development of an inducible pol III transcription system essentially requiring a mutated form of the TATA-binding protein.Nucleic acids research · 2001Article
- Yeast NC2 associates with the RNA polymerase II preinitiation complex and selectively affects transcription in vivo.Molecular and cellular biology · 2001Article
- Restoration of TATA-dependent transcription in a heat-inactivated extract of tobacco nuclei by recombinant TATA-binding protein (TBP) from tobacco.Plant molecular biology · 1997Article
- Species-specificity of rRNA gene transcription in plants manifested as a switch in RNA polymerase specificity.Nucleic acids research · 1996Article
- Role of TATA box sequence and orientation in determining RNA polymerase II/III transcription specificity.Nucleic acids research · 1996Article
- Mutations in the carboxy-terminal domain of TBP affect the synthesis of human immunodeficiency virus type 1 full-length and short transcripts similarly.Journal of virology · 1996Article
- An upstream U-snRNA gene-like promoter is required for transcription of the Arabidopsis thaliana 7SL RNA gene.Nucleic acids research · 1995Article
- Small nuclear RNA genes transcribed by either RNA polymerase II or RNA polymerase III in monocot plants share three promoter elements and use a strategy to regulate gene expression different from that used by their dicot plant counterparts.Molecular and cellular biology · 1994Article
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Authors and funding
3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Promoters of pol II and pol III transcribed U-snRNA genes in plants have identical sequence elements comprised of a -30 TATA box and an upstream sequence element (USE), located four or three helical turns upstream of the TATA box in pol II and pol III genes, respectively; it is this difference in element spacing that determines the RNA polymerase specificity of the gene. We are interested in identifying factors binding to U-snRNA gene promoters and their role in selection of RNA polymerase. In this work we have investigated possible differences in the activity of the two TATA binding proteins (TBPs) encoded by two different TBP genes of Arabidopsis. Using mutant TBPs with altered DNA binding specificity, similar to those described previously in yeast, we show that two Arabidopsis TBP isoforms are equally active with both pol II and pol III U-snRNA genes and with an mRNA gene transfected into plant protoplasts. In contrast to yeast, where modified TBP permits transcription only from promoters containing the TGTAAA mutant of the consensus (TATAAA) TATA element, altered Arabidopsis TBPs also suppress other TATA box mutants. Similar results were obtained with human and yeast TBP mutants expressed in plant cells. Interestingly, in several cases suppression of different TATA box mutants by altered TBPs was gene or RNA polymerase specific suggesting that assembly of TBP into specific complexes containing different TBP-associated factors may alter DNA binding specificity of the protein.
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