ArticleThe EMBO journal1993
Calmodulin is essential for assembling links necessary for exocytotic membrane fusion in Paramecium.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Calmodulin inMicroorganisms · 2022Review
- The Role of Calmodulin vs. Synaptotagmin in Exocytosis.Frontiers in molecular neuroscience · 2021Review
- Calmodulin and lipid binding to synaptobrevin regulates calcium-dependent exocytosis.The EMBO journal · 2002Article
- N-ethylmaleimide-sensitive factor is required to organize functional exocytotic microdomains in paramecium.Genetics · 2002Article
- The EF-hand Ca(2+)-binding protein p22 associates with microtubules in an N-myristoylation-dependent manner.Molecular biology of the cell · 1999Article
- Hormone-induced secretory and nuclear translocation of calmodulin: oscillations of calmodulin concentration with the nucleus as an integrator.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Genetic approach to regulated exocytosis using functional complementation in Paramecium: identification of the ND7 gene required for membrane fusion.Molecular biology of the cell · 1997Article
- Granule lattice protein 1 (Grl1p), an acidic, calcium-binding protein in Tetrahymena thermophila dense-core secretory granules, influences granule size, shape, content organization, and release but not protein sorting or condensation.The Journal of cell biology · 1996Article
- Protein phosphatase and kinase activities possibly involved in exocytosis regulation in Paramecium tetraurelia.The Biochemical journal · 1996Article
- Distinct effects of alpha-SNAP, 14-3-3 proteins, and calmodulin on priming and triggering of regulated exocytosis.The Journal of cell biology · 1995Article
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Authors and funding
4 authors.
Funding
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Abstract
Calmodulin has long been suspected to be involved in calcium-regulated exocytosis but its precise site(s) of action has not yet been identified. In Paramecium, a genetic approach to the problem is possible as in vivo-selected mutations in the calmodulin gene that prevent the activation of some channels have been characterized. Three of these calmodulin mutants were examined for exocytotic capacity and the mutant cam1 was found to be defective for exocytosis at 35 degrees C. The loss of exocytotic capacity in cam1 cells can be restored by transformation with the wild-type calmodulin gene, demonstrating that its exocytotic lesion is indeed due to the mutation in the calmodulin gene. The cam1 mutant displays abnormal exocytotic sites at the non-permissive temperature: it lacks the links ('rosettes' of intramembranous particles in the plasma membrane and the fibrous 'connecting material') which normally connect plasma and trichocyst membranes. Upon shift of cam1 cells from the permissive to a non-permissive temperature, performed sites remain functional. These results demonstrate that calmodulin is necessary for the assembly of these links at the exocytotic site. These results do not, however, exclude the possibility of calmodulin also being involved in Ca(2+)-dependent steps of the stimulus-exocytosis coupling.
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