ArticlePlant molecular biology1993
Secretion of a functional single-chain Fv protein in transgenic tobacco plants and cell suspension cultures.
Article in Plant molecular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed.
- Expression of synthetic human tropoelastin (hTE) protein in Nicotiana tabacum.GM crops & food · 2015Article
- Expression of recombinant antibody (single chain antibody fragment) in transgenic plant Nicotiana tabacum cv. Xanthi.Molecular biology reports · 2013Article
- Molecular Pharming: future targets and aspirations.Human vaccines · 2011Review
- Soybean peroxidase propeptides are functional signal peptides and increase the yield of a foreign protein.Plant cell reports · 2010Article
- Production of human serum albumin by sugar starvation induced promoter and rice cell culture.Transgenic research · 2005Article
- Plant cell cultures for the production of recombinant proteins.Nature biotechnology · 2004Review
- Rhizosecretion of recombinant proteins from plant hairy roots.Plant cell reports · 2003Article
- Rhizosecretion of a monoclonal antibody protein complex from transgenic tobacco roots.Plant molecular biology · 2003Article
- Expression of anti human IL-4 and IL-6 scFvs in transgenic tobacco plants.Plant molecular biology · 2003Article
- Targeted expression of human serum albumin to potato tubers.Transgenic research · 2002Article
- Antibody-based resistance to plant pathogens.Transgenic research · 2001Review
- Assembly and plasma membrane targeting of recombinant immunoglobulin chains in plants with a murine immunoglobulin transmembrane sequence.Plant molecular biology · 2001Article
- Molecular farming of pharmaceutical proteins.Transgenic research · 2000Review
- Transient expression of a tumor-specific single-chain fragment and a chimeric antibody in tobacco leaves.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Apoplastic and cytosolic expression of full-size antibodies and antibody fragments in Nicotiana tabacum.Transgenic research · 1999Article
- Use of plant roots for phytoremediation and molecular farming.Proceedings of the National Academy of Sciences of the United States of America · 1999Review
- Type II fish antifreeze protein accumulation in transgenic tobacco does not confer frost resistance.Transgenic research · 1999Article
- Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco plants.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Rice cell culture as an alternative production system for functional diagnostic and therapeutic antibodies.Transgenic research · 1999Article
- Engineering of a single-chain variable-fragment (scFv) antibody specific for the stolbur phytoplasma (Mollicute) and its expression in escherichia coli and tobacco plantsApplied and environmental microbiology · 1998Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A synthetic gene encoding an anti-phytochrome single-chain Fv (scFv) antibody bearing an N-terminal signal peptide has been used to transform tobacco plants. Immunoblot analysis showed that transformed plants accumulate high levels of scFv protein, accounting for up to 0.5% of the total soluble protein fraction, which could be extracted by simple infiltration and centrifugation of leaf tissue. A substantial proportion of the scFv protein extracted in this way was found to possess antigen-binding activity. Callus cell suspension cultures derived from transformed plants secrete functional scFv protein into the surrounding medium. Compared with the levels of scFv protein observed in plants expressing the native scFv gene, the incorporation of an N-terminal signal peptide, to target the scFv to the apoplast, results in elevated accumulation of the protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.