ArticlePlant molecular biology1993
Single-copy T-DNA insertions in Arabidopsis are the predominant form of integration in root-derived transgenics, whereas multiple insertions are found in leaf discs.
Article in Plant molecular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Binary vector origin predictably determinesbioRxiv : the preprint server for biology · 2025Article
- Genomic consequences associated with Agrobacterium-mediated transformation of plants.The Plant journal : for cell and molecular biology · 2024Review
- T-LOCked in: Identifying T-DNA insertions in plant genomes.Plant physiology · 2022Article
- T-LOC: A comprehensive tool to localize and characterize T-DNA integration sites.Plant physiology · 2022Article
- Gene targeting in polymerase theta-deficient Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2022Article
- Agrobacterium-mediated plant transformation: biology and applications.The arabidopsis book · 2017Article
- Comparison of DNA walking methods for isolation of transgene-flanking regions in GM potato.Molecular biotechnology · 2011Article
- Generation of backbone-free, low transgene copy plants by launching T-DNA from the Agrobacterium chromosome.Plant physiology · 2010Article
- An inducible targeted tagging system for localized saturation mutagenesis in Arabidopsis.Plant physiology · 2005Article
- Two-fold differences are the detection limit for determining transgene copy numbers in plants by real-time PCR.BMC biotechnology · 2004Article
- Transgene organisation in potato after particle bombardment-mediated (co-)transformation using plasmids and gene cassettes.Transgenic research · 2003Article
- Genetic transformation of conifers and its application in forest biotechnology.Plant cell reports · 2003Review
- Agrobacterium-mediated plant transformation: the biology behind the "gene-jockeying" tool.Microbiology and molecular biology reviews : MMBR · 2003Review
- Molecular structure and regulatory potential of a T-DNA integration site in petunia.Transgenic research · 2003Article
- A high-throughput Arabidopsis reverse genetics system.The Plant cell · 2002Article
- Plants as bioreactors for protein production: avoiding the problem of transgene silencing.Plant molecular biology · 2000Review
- Bacterial conjugation protein MobA mediates integration of complex DNA structures into plant cells.Journal of bacteriology · 1999Article
- Transgene organization in rice engineered through direct DNA transfer supports a two-phase integration mechanism mediated by the establishment of integration hot spots.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Frequent collinear long transfer of DNA inclusive of the whole binary vector during Agrobacterium-mediated transformation.Plant molecular biology · 1997Article
- Characterisation of a new allele of pale cress and its role in greening in Arabidopsis thaliana.Molecular & general genetics : MGG · 1996Article
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5 authors.
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Abstract
Different patterns of T-DNA integration in Arabidopsis were obtained that depended on whether a root or a leaf-disc transformation method was used. An examination of 82 individual transgenic Arabidopsis plants, derived from 15 independent Agrobacterium-mediated transformations in which different cointegrate and binary constructs were used, indicated that the transformation method had a significant influence on the type and copy number of T-DNA integration events. Southern hybridizations showed that most of the transgenic plants produced by a leaf-disc method contained multiple T-DNA insertions (89%), the majority of which were organized as right-border inverted repeat structures (58%). In contrast, a root transformation method mostly resulted in single T-DNA insertions (64%), with fewer right-border inverted repeats (38%). The transformation vectors, including cointegrate and binary types, and the plant selectable markers, hygromycin phosphotransferase and dihydrofolate reductase, did not appear to influence the T-DNA integration patterns.
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