ArticlePlant molecular biology1993
Cytosine deaminase as a negative selective marker for Arabidopsis.
Article in Plant molecular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 47 citations in OpenAlex.
- A bacterial gene codA encoding cytosine deaminase is an effective conditional negative selectable marker in Glycine max.Plant cell reports · 2015Article
- A Universal Positive-Negative Selection System for Gene Targeting in Plants Combining an Antibiotic Resistance Gene and Its Antisense RNA.Plant physiology · 2015Article
- The codA gene as a negative selection marker in Citrus.SpringerPlus · 2015Article
- Precise marker excision system using an animal-derived piggyBac transposon in plants.The Plant journal : for cell and molecular biology · 2014Article
- Transposition-based plant transformation.Plant physiology · 2007Article
- Modification of endogenous natural genes by gene targeting in rice and other higher plants.Plant molecular biology · 2005Article
- Crop improvement through modification of the plant's own genome.Plant physiology · 2004Article
- The HY2 gene as an efficient marker for transposon excision in Arabidopsis.Molecular genetics and genomics : MGG · 2003Article
- Purine and pyrimidine nucleotide synthesis and metabolism.The arabidopsis book · 2002Article
- Arabidopsis mutants deficient in T-DNA integration.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Variable expression of the herpes simplex virus thymidine kinase gene in Nicotiana tabacum affects negative selection.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1995Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytosine deaminase (CD), produced by prokaryotes but not by higher eukaryotes including plants, deaminates cytosine to uracil. The enzyme likewise converts 5-fluorocytosine (5FC), which by itself is not toxic, to 5-fluorouracil (5FU), which is toxic. The Escherichia coli codA-coding sequence encoding CD, together with appropriate regulatory elements, was introduced into Arabidopsis. Neither untransformed controls, nor transgenic plants expressing no CD mRNA, were sensitive to 5FC. Conversely, for most transgenic plants expressing CD mRNA, in the presence of 5FC calli and seedlings failed to proliferate, and seeds failed to germinate. A few transgenic plants with many codA copies expressed less CD mRNA and remained insensitive to 5FC, which likely reflected epigenetic repeat-induced gene silencing. Thus 5FC, presumably through conversion by the enzyme to 5FU, can be used to select against plants that express CD.
Indexed as
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.