ArticleProceedings of the National Academy of Sciences of the United States of America1993
Evidence for myosin motors on organelles in squid axoplasm.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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Who cites it
26 citing papers in PubMed, 83 citations in OpenAlex.
- The Giant Axon of the Squid: A Simple System for Axonal Transport Studies.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Quantitative measurements and modeling of cargo-motor interactions during fast transport in the living axon.Physical biology · 2012Article
- ApoE isoform-specific regulation of regeneration in the peripheral nervous system.Human molecular genetics · 2011Article
- A peptide zipcode sufficient for anterograde transport within amyloid precursor protein.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Fast anterograde transport of herpes simplex virus: role for the amyloid precursor protein of alzheimer's disease.Aging cell · 2003Article
- The role of the cytoskeleton in the life cycle of viruses and intracellular bacteria: tracks, motors, and polymerization machines.Current drug targets. Infectious disorders · 2002Article
- Association of a nonmuscle myosin II with axoplasmic organelles.Molecular biology of the cell · 2002Article
- Microsequencing of myosins for PCR primer design.Methods in molecular biology (Clifton, N.J.) · 2001Review
- Retrograde axonal transport of herpes simplex virus: evidence for a single mechanism and a role for tegument.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Squid axoplasm supports the retrograde axonal transport of herpes simplex virus.The Biological bulletin · 1999Article
- Regulation of melanosome movement in the cell cycle by reversible association with myosin V.The Journal of cell biology · 1999Article
- Association of actin filaments with axonal microtubule tracts.Journal of neurocytology · 1999Article
- Visualization of melanosome dynamics within wild-type and dilute melanocytes suggests a paradigm for myosin V function In vivo.The Journal of cell biology · 1998Article
- Primary peptide sequences from squid muscle and optic lobe myosin IIs: a strategy to identify an organelle myosin.Cell biology international · 1998Article
- Subcellular localization of myosin-V in the B16 melanoma cells, a wild-type cell line for the dilute gene.Molecular biology of the cell · 1997Article
- Brain myosin V is a synaptic vesicle-associated motor protein: evidence for a Ca2+-dependent interaction with the synaptobrevin-synaptophysin complex.The Journal of cell biology · 1997Article
- Unconventional myosins in inner-ear sensory epithelia.The Journal of cell biology · 1997Article
- Immunochemical analysis of kinesin light chain function.Molecular biology of the cell · 1997Article
- Actin-based organelle movement.Experientia · 1996Review
- Actin and myosin function in directed vacuole movement during cell division in Saccharomyces cerevisiae.The Journal of cell biology · 1996Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Squid axoplasm has proved a rich source for the identification of motors involved in organelle transport. Recently, squid axoplasmic organelles have been shown to move on invisible tracks that are sensitive to cytochalasin, suggesting that these tracks are actin filaments. Here, an assay is described that permits observation of organelles moving on unipolar actin bundles. This assay is used to demonstrate that axoplasmic organelles move on actin filaments in the barbed-end direction, suggesting the presence of a myosin motor on axoplasmic organelles. Indeed, axoplasm contains actin-dependent ATPase activity, and a pan-myosin antibody recognized at least four bands in Western blots of axoplasm. An approximately 235-kDa band copurified in sucrose gradients with KI-extracted axoplasmic organelles, and the myosin antibody stained the organelle surfaces by immunogold electron microscopy. The myosin is present on the surface of at least some axoplasmic organelles and thus may be involved in their transport through the axoplasm, their movement through the cortical actin in the synapse, or some other aspect of axonal function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.