ArticleProceedings of the National Academy of Sciences of the United States of America1993
Use of yeast artificial chromosomes (YACs) for studying control of gene expression: correct regulation of the genes of a human beta-globin locus YAC following transfer to mouse erythroleukemia cell lines.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- One Yeast, Sixteen Synthetic Chromosomes, Infinite Possibilities.Yeast (Chichester, England) · 2026Review
- An intergenic non-coding RNA promoter required for histone modifications in the human β-globin chromatin domain.PloS one · 2019Article
- A cell-based high-throughput screen for novel chemical inducers of fetal hemoglobin for treatment of hemoglobinopathies.PloS one · 2014Article
- Mi2β is required for γ-globin gene silencing: temporal assembly of a GATA-1-FOG-1-Mi2 repressor complex in β-YAC transgenic mice.PLoS genetics · 2012Article
- Induction of Fetal Hemoglobin In Vivo Mediated by a Synthetic γ-Globin Zinc Finger Activator.Anemia · 2012Article
- MBD2 contributes to developmental silencing of the human ε-globin gene.Blood cells, molecules & diseases · 2011Article
- Design of embedded chimeric peptide nucleic acids that efficiently enter and accurately reactivate gene expression in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Cell to cell transfer of the chromatin-packaged human beta-globin gene cluster.Nucleic acids research · 2010Article
- The higher structure of chromatin in the LCR of the beta-globin locus changes during development.Journal of molecular biology · 2009Article
- Silencing of Agamma-globin gene expression during adult definitive erythropoiesis mediated by GATA-1-FOG-1-Mi2 complex binding at the -566 GATA site.Molecular and cellular biology · 2008Article
- Investigations of a human embryonic globin gene silencing element using YAC transgenic mice.Experimental biology and medicine (Maywood, N.J.) · 2006Article
- Synergistic and additive properties of the beta-globin locus control region (LCR) revealed by 5'HS3 deletion mutations: implication for LCR chromatin architecture.Molecular and cellular biology · 2005Article
- Silencing of Fshr occurs through a conserved, hypersensitive site in the first intron.Molecular endocrinology (Baltimore, Md.) · 2005Article
- Control of globin gene expression during development and erythroid differentiation.Experimental hematology · 2005Review
- Mutation of a transcriptional motif of a distant regulatory element reduces the expression of embryonic and fetal globin genes.Human molecular genetics · 2003Article
- Identification of a YAC from 16q24 carrying a senescence gene for breast cancer cells.Oncogene · 2000Article
- The absence of enhancer competition between Igf2 and H19 following transfer into differentiated cells.Molecular and cellular biology · 1998Article
- Position-independent expression of a human nerve growth factor-luciferase reporter gene cloned on a yeast artificial chromosome vector.Nucleic acids research · 1998Article
- A dicistronic construct allows easy detection of human CFTR expression from YAC DNA in human cells.Nucleic acids research · 1997Article
- Mutation of gene-proximal regulatory elements disrupts human epsilon-, gamma-, and beta-globin expression in yeast artificial chromosome transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
We demonstrate that transfer of a yeast artificial chromosome (YAC) containing 230 kb of the human beta-globin locus into mouse erythroleukemia cells by fusion results in correct developmental regulation of the human beta-like globin genes. Additionally, we show that early after hybrid formation, human embryonic epsilon- and fetal gamma-globin genes are coexpressed with the adult beta gene but that after 10-20 weeks in culture, globin gene expression switches to predominantly adult. Thus, in contrast to shorter gene constructs, the globin genes of the beta-globin locus YAC are regulated like the chromosomal globin genes. These results indicate that transfer of YACs into established cell lines can be used for the analysis of the developmental control of multigenic and developmentally regulated human loci.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.