ArticleProceedings of the National Academy of Sciences of the United States of America1993
Cellular epigenetics: effects of passage history on competence of cells for "spontaneous" transformation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Article
- Interlaboratory evaluation of a digital holographic microscopy-based assay for label-free in vitro cytotoxicity testing of polymeric nanocarriers.Drug delivery and translational research · 2022Article
- Delineating the Effects of Passaging and Exposure in a Longitudinal Study of Arsenic-Induced Squamous Cell Carcinoma in a HaCaT Cell Line Model.Toxicological sciences : an official journal of the Society of Toxicology · 2022Article
- Genome based cell population heterogeneity promotes tumorigenicity: the evolutionary mechanism of cancer.Journal of cellular physiology · 2009Article
- Article
- Clonal dynamics of progressive neoplastic transformation.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- The cellular ecology of progressive neoplastic transformation: a clonal analysis.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Immunobead filtration: a novel approach for the isolation and propagation of tumor cells.The American journal of pathology · 1997Article
- Neoplastic development: paradoxical relation between impaired cell growth at low population density and excessive growth at high density.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- A critical test of the role of population density in producing transformation.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Experimental control of neoplastic progression in cell populations: Foulds' rules revisited.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Selective nature of phorbol 12-myristate 13-acetate-induced neoplastic transformation in NIH 3T3 cells.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Cellular epigenetics: control of the size, shape, and spatial distribution of transformed foci by interactions between the transformed and nontransformed cells.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Cellular epigenetics: topochronology of progressive "spontaneous" transformation of cells under growth constraint.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
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1 author.
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Abstract
"Spontaneous" neoplastic transformation of cells in culture has been shown to be an adaptive response to moderate physiological constraints such as contact inhibition and lowered concentrations of serum. These are the same constraints that promote normal differentiation of a variety of cell types. Because both normal and neoplastic development represent enduring changes in response to constraint, spontaneous transformation can be considered a form of epigenesis. This phenomenon permits the use of established cell lines in developing general epigenetic principles with all of the advantages of manipulation, efficient cloning, and quantification afforded by such systems. Being environmentally sensitive, however, the responsive capacity of cell lines is subject to fluctuation and long-term modification. I therefore set out to define the conditions used in maintaining cells that influence their competence to undergo transformation with the aim of controlling that competence. It proved to be sensitive to both the population density and frequency of three different repetitive passage regimens. Both the saturation density of the cells and their capacity to produce transformed foci varied initially in different ways within the same regimen in two consecutive passage series starting from the same frozen stock, but both parameters eventually settled down to values characteristic of each of the three regimens. As a result, three sublines were developed of high, intermediate, and low competence for transformation. Each of the sublines has special advantages for studying different aspects of epigenetic change. In the process of developing the sublines, a number of observations were made that reinforce the epigenetic nature of neoplastic transformation and are consistent with the concept of progressive state selection as the basis of the change. That concept can be considered a first step in the formal analysis of epigenesis.
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