ArticleProceedings of the National Academy of Sciences of the United States of America1993
Mechanistic aspects of genome-wide demethylation in the preimplantation mouse embryo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 138 citations in OpenAlex.
- Metabolic Plasticity in Embryogenesis Throughout the Lens of NADMethods in molecular biology (Clifton, N.J.) · 2026Review
- TET2 and TET3 loss disrupts small intestine differentiation and homeostasis.Nature communications · 2023Article
- TRIM28 Controls Genomic Imprinting through Distinct Mechanisms during and after Early Genome-wide Reprogramming.Cell reports · 2015Article
- Diversity of two forms of DNA methylation in the brain.Frontiers in genetics · 2014Article
- DNA methylation program during development.Frontiers in biology · 2012Article
- DNA methylation: an epigenetic risk factor in preterm birth.Reproductive sciences (Thousand Oaks, Calif.) · 2012Review
- Localization of DNA methyltransferase-1 during oocyte differentiation, in vitro maturation and early embryonic development in cow.European journal of histochemistry : EJH · 2009Article
- Evidence for conserved DNA and histone H3 methylation reprogramming in mouse, bovine and rabbit zygotes.Epigenetics & chromatin · 2008Article
- Diet and the epigenetic (re)programming of phenotypic differences in behavior.Brain research · 2008Review
- 5-Methylcytosine DNA glycosylase participates in the genome-wide loss of DNA methylation occurring during mouse myoblast differentiation.Nucleic acids research · 2001Article
- The battle of the sexes after fertilization: behaviour of paternal and maternal chromosomes in the early mammalian embryo.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2001Review
- DNA methylation is a reversible biological signal.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- DNA demethylation.Proceedings of the National Academy of Sciences of the United States of America · 1999Review
- Evidence that protein binding specifies sites of DNA demethylation.Molecular and cellular biology · 1999Article
- Chromosome methylation patterns during mammalian preimplantation development.Genes & development · 1998Article
- Regulation of X-chromosome inactivation in development in mice and humans.Microbiology and molecular biology reviews : MMBR · 1998Review
- An embryonic demethylation mechanism involving binding of transcription factors to replicating DNA.The EMBO journal · 1998Article
- Embryonic germ cells induce epigenetic reprogramming of somatic nucleus in hybrid cells.The EMBO journal · 1997Article
- Structure of the imprinted mouse Snrpn gene and establishment of its parental-specific methylation pattern.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Comparison of DNA methylation patterns among mouse cell lines by restriction landmark genomic scanning.Molecular and cellular biology · 1994Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene-specific methylation patterns in mammals play a role in a variety of biological processes in the embryo and adult tissues. These patterns are established during embryo development by a process that involves genome-wide demethylation in the morula and de novo methylation in the pregastrula. To elucidate the mechanism of demethylation in the early mouse embryo, we have injected mouse zygotes with gene sequences that were methylated in vitro by Hpa II methylase and analyzed the methylation status of specific sites in blastocyst DNA. Because it had been propagated in Escherichia coli, the DNA used for these injections was also methylated at adenine residues in GATC sites. This allowed us to eliminate fully methylated, unintegrated DNA by Dpn I digestion and fully unmethylated, integrated DNA that underwent several rounds of replication by Mbo I digestion. The integrated, originally injected DNA strands were in a hemimethylated state and survived this treatment. The methylation status of Hpa II sites in these molecules was analyzed by Hpa II digestion of the genomic DNA isolated from blastocysts, followed by PCR amplification using appropriate primers. The results demonstrate that demethylation is achieved by an active mechanism and that specific sites in imprinted genes escape demethylation, maintaining a methylated state throughout preimplantation development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.