ArticleMolecular and cellular biology1993
A regulatory element in the CHA1 promoter which confers inducibility by serine and threonine on Saccharomyces cerevisiae genes.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 30 citations in OpenAlex.
- Modulators of a robust and efficient metabolism: Perspective and insights from the Rid superfamily of proteins.Advances in microbial physiology · 2023Review
- Absence of MMF1 disrupts heme biosynthesis by targeting Hem1pin Saccharomyces cerevisiae.Yeast (Chichester, England) · 2021Article
- Modular and Molecular Optimization of a LOV (Light-Oxygen-Voltage)-Based Optogenetic Switch in Yeast.International journal of molecular sciences · 2021Article
- Independent evolution of transcript abundance and gene regulatory dynamics.Genome research · 2020Article
- Article
- Genome-wide association of mediator and RNA polymerase II in wild-type and mediator mutant yeast.Molecular and cellular biology · 2015Article
- Sphingoid bases and the serine catabolic enzyme CHA1 define a novel feedforward/feedback mechanism in the response to serine availability.The Journal of biological chemistry · 2012Article
- Stitching together multiple data dimensions reveals interacting metabolomic and transcriptomic networks that modulate cell regulation.PLoS biology · 2012Article
- Ammonia-specific regulation of Gln3 localization in Saccharomyces cerevisiae by protein kinase Npr1.The Journal of biological chemistry · 2006Article
- A fungal family of transcriptional regulators: the zinc cluster proteins.Microbiology and molecular biology reviews : MMBR · 2006Review
- Chromatin-mediated transcriptional regulation by the yeast architectural factors NHP6A and NHP6B.The EMBO journal · 2000Article
- Stp1p, Stp2p and Abf1p are involved in regulation of expression of the amino acid transporter gene BAP3 of Saccharomyces cerevisiae.Nucleic acids research · 2000Article
- Activation of silent replication origins at autonomously replicating sequence elements near the HML locus in budding yeast.Molecular and cellular biology · 1999Article
- Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex RSC.The EMBO journal · 1999Article
- Article
- Identification and characterization of MAE1, the Saccharomyces cerevisiae structural gene encoding mitochondrial malic enzyme.Journal of bacteriology · 1998Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CHA1 of Saccharomyces cerevisiae is the gene for the catabolic L-serine (L-threonine) dehydratase, which is responsible for biodegradation of serine and threonine. We have previously shown that expression of the CHA1 gene is transcriptionally induced by serine and threonine. Northern (RNA) analysis showed that the additional presence of good nitrogen sources affects induction. This may well be due to inducer exclusion. To identify interactions of cis-acting elements with trans activators of the CHA1 promoter, we performed band shift assays of nuclear protein extracts with CHA1 promoter fragments. By this approach, we identified a protein-binding site of the CHA1 promoter. The footprint of this protein contains the ABF1-binding site consensus sequence. This in vitro binding activity is present irrespectively of CHA1 induction. By deletion analysis, two other elements of the CHA1 promoter, UAS1CHA and UAS2CHA, which are needed for induction of the CHA1 gene were identified. Each of the two sequence elements is sufficient to confer serine and threonine induction upon the CYC1 promoter when substituting its upstream activating sequence. Further, in a cha4 mutant strain which is unable to grow with serine or threonine as the sole nitrogen source, the function of UAS1CHA, as well as that of UAS2CHA, is obstructed.
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