ArticleMolecular and cellular biology1993
Interaction of the intron-encoded mobility endonuclease I-PpoI with its target site.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Pathways of rDNA copy number homeostasis in Schizosaccharomyces pombe.G3 (Bethesda, Md.) · 2026Article
- Efficient Nuclease-Directed Integration of Lentivirus Vectors into the Human Ribosomal DNA Locus.Molecular therapy : the journal of the American Society of Gene Therapy · 2020Article
- The effects of manipulating levels of replication initiation factors on origin firing efficiency in yeast.PLoS genetics · 2019Article
- 5'-Cytosine-phosphoguanine (CpG) methylation impacts the activity of natural and engineered meganucleases.The Journal of biological chemistry · 2012Article
- A new method to efficiently induce a site-specific double-strand break in the fission yeast Schizosaccharomyces pombe.Yeast (Chichester, England) · 2012Article
- RNA aptamers that functionally interact with green fluorescent protein and its derivatives.Nucleic acids research · 2012Article
- Pushing the limits of what is achievable in protein-DNA docking: benchmarking HADDOCK's performance.Nucleic acids research · 2010Article
- Knocking down gene function with an RNA aptamer expressed as part of an intron.Nucleic acids research · 2010Article
- Homing endonucleases: from basics to therapeutic applications.Cellular and molecular life sciences : CMLS · 2010Review
- Targeting the X chromosome during spermatogenesis induces Y chromosome transmission ratio distortion and early dominant embryo lethality in Anopheles gambiae.PLoS genetics · 2008Article
- Thermodynamics of DNA target site recognition by homing endonucleases.Nucleic acids research · 2007Article
- Altered target site specificity variants of the I-PpoI His-Cys box homing endonuclease.Nucleic acids research · 2007Article
- DNA binding and cleavage by the periplasmic nuclease Vvn: a novel structure with a known active site.The EMBO journal · 2003Article
- Transcription and tyranny in the nucleolus: the organization, activation, dominance and repression of ribosomal RNA genes.The arabidopsis book · 2002Article
- Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility.Nucleic acids research · 2001Review
- I-PpoI, the endonuclease encoded by the group I intron PpLSU3, is expressed from an RNA polymerase I transcript.Molecular and cellular biology · 1998Article
- Crystallization and preliminary X-ray studies of I-PpoI: a nuclear, intron-encoded homing endonuclease from Physarum polycephalum.Protein science : a publication of the Protein Society · 1997Article
- Homing endonucleases: keeping the house in order.Nucleic acids research · 1997Review
- Profile of the DNA recognition site of the archaeal homing endonuclease I-DmoI.Nucleic acids research · 1997Article
- Single-site manipulation of tomato chromosomes in vitro and in vivo using Cre-lox site-specific recombination.Plant molecular biology · 1996Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Endonucleases encoded by mobile group I introns are highly specific DNases that induce a double-strand break near the site to which the intron moves. I-PpoI from the acellular slime mold Physarum polycephalum mediates the mobility of intron 3 (Pp LSU 3) in the extrachromosomal nuclear ribosomal DNA of this organism. We showed previously that cleavage by I-PpoI creates a four-base staggered cut near the point of intron insertion. We have now characterized several further properties of the endonuclease. As determined by deletion analysis, the minimal target site recognized by I-PopI was a sequence of 13 to 15 bp spanning the cleavage site. The purified protein behaved as a globular dimer in sedimentation and gel filtration. In gel mobility shift assays in the presence of EDTA, I-PpoI formed a stable and specific complex with DNA, dissociating with a half-life of 45 min. By footprinting and interference assays with methidiumpropyl-EDTA-iron(II), I-PpoI contacted a 22- to 24-bp stretch of DNA. The endonuclease protected most of the purines found in both the major and minor grooves of the DNA helix from modification by dimethyl sulfate (DMS). However, the reactivity to DMS was enhanced at some purines, suggesting that binding leads to a conformational change in the DNA. The pattern of DMS protection differed fundamentally in the two partially symmetrical halves of the recognition sequence.
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