ArticleMolecular & general genetics : MGG1993
Functional expression of the transcriptional activator Opaque-2 of Zea mays in transformed yeast.
Article in Molecular & general genetics : MGG, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- Identification and characterization of lysine-rich proteins and starch biosynthesis genes in the opaque2 mutant by transcriptional and proteomic analysis.BMC plant biology · 2013Article
- The Zea mays mutants opaque-2 and opaque-7 disclose extensive changes in endosperm metabolism as revealed by protein, amino acid, and transcriptome-wide analyses.BMC genomics · 2011Article
- Chromatin and DNA modifications in the Opaque2-mediated regulation of gene transcription during maize endosperm development.The Plant cell · 2009Article
- Wild-type opaque2 and defective opaque2 polypeptides form complexes in maize endosperm cells and bind the opaque2-zein target site.Plant physiology · 2007Article
- Transcriptional and biochemical regulation of a novel Arabidopsis thaliana bifunctional aspartate kinase-homoserine dehydrogenase gene isolated by functional complementation of a yeast hom6 mutant.Plant molecular biology · 2003Article
- An improved genetic model generates high-resolution mapping of QTL for protein quality in maize endosperm.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Genetic analysis of amino acid accumulation in opaque-2 maize endosperm.Plant physiology · 2001Article
- Aspartate kinase 2. A candidate gene of a quantitative trait locus influencing free amino acid content in maize endosperm.Plant physiology · 2001Article
- Tobacco and Arabidiopsis SLT1 mediate salt tolerance of yeast.Plant molecular biology · 2001Article
- Molecular characterization and expression study of a histidine auxotrophic mutant (his1-) of Nicotiana plumbaginifolia.Plant molecular biology · 2001Article
- Specific combinations of zein genes and genetic backgrounds influence the transcription of the heavy-chain zein genes in maize opaque-2 endosperms.Plant physiology · 2000Article
- The DapA gene encoding the lysine biosynthetic enzyme dihydrodipicolinate synthase from Coix lacryma-jobi: cloning, characterization, and expression analysis.Plant molecular biology · 1999Article
- The role of opaque2 in the control of lysine-degrading activities in developing maize endosperm.The Plant cell · 1999Article
- The wheat transcriptional activator SPA: a seed-specific bZIP protein that recognizes the GCN4-like motif in the bifactorial endosperm box of prolamin genes.The Plant cell · 1997Article
- The import receptor for the peroxisomal targeting signal 2 (PTS2) in Saccharomyces cerevisiae is encoded by the PAS7 gene.The EMBO journal · 1996Article
- The transcriptional activator Opaque-2 controls the expression of a cytosolic form of pyruvate orthophosphate dikinase-1 in maize endosperms.Molecular & general genetics : MGG · 1996Article
- The maize transcription factor Opaque-2 activates a wheat glutenin promoter in plant and yeast cells.Plant molecular biology · 1995Article
- Insertion mutations at the maize Opaque2 locus induced by transposable element families Ac, En/Spm and Bg.Molecular & general genetics : MGG · 1995Article
- Regulation of Lysine and Threonine Synthesis.The Plant cell · 1995Article
- A novel zinc finger protein encoded by a couch potato homologue from Solanum tuberosum enables a sucrose transport-deficient yeast strain to grow on sucrose.Molecular & general genetics : MGG · 1995Article
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7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this research was to determine whether the structural homology between the O2 gene, a maize transcriptional activator, and the GCN4 gene, a yeast transcriptional factor, is reflected at the level of function. The O2 cDNA was cloned in the yeast expression vector pEMBLyex4 under the control of a hybrid inducible promoter, and used to transform the yeast Saccharomyces cerevisiae. Transformed yeast cells produced O2 mRNA and a polypeptide immunoreactive with anti-O2 antibodies during growth in galactose. The heterologous protein was correctly translocated into the yeast nuclei, as demonstrated by immunofluorescence, indicating that the nuclear targeting sequences of maize are recognized by yeast cells. Further experiments demonstrated the ability of O2 to rescue a gcn4 mutant grown in the presence of aminotriazole, an inhibitor of the HIS3 gene product, suggesting that O2 activates the HIS3 gene, gene normally under control of GCN4. It was shown that the O2 protein is able to trans-activate the HIS4 promoter in yeast cells and binds to it in vitro. The sequence protected by O2, TGACTC, is also the binding site for GCN4. Finally, the expression of O2 protein in yeast did not produce alterations during batch growth at 30 degrees C, while transformants expressing O2 protein showed a conditionally lethal phenotype when grown in galactose at 36 degrees C; this phenotype mimics the behaviour of gcd mutants. The results support the idea that basic mechanisms of transcription control have been highly conserved in eukaryotes.
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