ArticleThe Journal of cell biology1993
Multiple regions of NSR1 are sufficient for accumulation of a fusion protein within the nucleolus.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 61 citations in OpenAlex.
- BrMYB116 transcription factor enhances Cd stress tolerance by activating FIT3 in yeast and Chinese cabbage.Frontiers in plant science · 2024Article
- Targeting of Hmo1 to subcompartments of the budding yeast nucleolus.Molecular biology of the cell · 2023Article
- Shaping the Nascent Ribosome: AAA-ATPases in Eukaryotic Ribosome Biogenesis.Biomolecules · 2019Review
- Impact of histone H4K16 acetylation on the meiotic recombination checkpoint inMicrobial cell (Graz, Austria) · 2016Article
- Acclimation of Saccharomyces cerevisiae to low temperature: a chemostat-based transcriptome analysis.Molecular biology of the cell · 2007Article
- Protein pyrophosphorylation by inositol pyrophosphates is a posttranslational event.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Arabidopsis nucleolin affects plant development and patterning.Plant physiology · 2007Article
- Article
- Localization in the nucleolus and coiled bodies of protein subunits of the ribonucleoprotein ribonuclease P.The Journal of cell biology · 1999Article
- Nuclear and nucleolar targeting of human ribosomal protein S25: common features shared with HIV-1 regulatory proteins.Oncogene · 1999Article
- Crm1p mediates regulated nuclear export of a yeast AP-1-like transcription factor.The EMBO journal · 1998Article
- Nucleolar localization elements in U8 snoRNA differ from sequences required for rRNA processing.RNA (New York, N.Y.) · 1998Article
- The Drosophila gene for antizyme requires ribosomal frameshifting for expression and contains an intronic gene for snRNP Sm D3 on the opposite strand.Molecular and cellular biology · 1998Article
- cDNA cloning and characterization of the human U3 small nucleolar ribonucleoprotein complex-associated 55-kilodalton protein.Molecular and cellular biology · 1998Article
- Article
- Topogenesis of a nucleolar protein: determination of molecular segments directing nucleolar association.Molecular biology of the cell · 1997Article
- An abundant nucleolar phosphoprotein is associated with ribosomal DNA in Tetrahymena macronuclei.Molecular biology of the cell · 1997Article
- Ultraviolet-induced movement of the human DNA repair protein, Xeroderma pigmentosum type G, in the nucleus.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Nucleoplasmic and nucleolar distribution of the adenovirus IVa2 gene product.Journal of virology · 1996Article
- Developmental and cell cycle regulation of alfalfa nucMs1, a plant homolog of the yeast Nsr1 and mammalian nucleolin.The Plant cell · 1996Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
NSR1, a 67-kD nucleolar protein, was originally identified in our laboratory as a nuclear localization signal binding protein, and has subsequently been found to be involved in ribosome biogenesis. NSR1 has three regions: an acidic/serine-rich NH2 terminus, two RNA recognition motifs, and a glycine/arginine-rich COOH terminus. In this study we show that NSR1 itself has a bipartite nuclear localization sequence. Deletion of either basic amino acid stretch results in the mislocation of NSR1 to the cytoplasm. We further demonstrate that either of two regions, the NH2 terminus or both RNA recognition motifs, are sufficient to localize a bacterial protein, beta-galactosidase, to the nucleolus. Intensive deletion analysis has further defined a specific acidic/serine-rich region within the NH2 terminus as necessary for nucleolar accumulation rather than nucleolar targeting. In addition, deletion of either RNA recognition motif or point mutations in one of the RNP consensus octamers results in the mislocalization of a fusion protein within the nucleus. Although the glycine/arginine-rich region in the COOH terminus is not sufficient to bring beta-galactosidase to the nucleolus, our studies show that this domain is necessary for nucleolar accumulation when an RNP consensus octamer in one of the RNA recognition motifs is mutated. Our findings are consistent with the notion that nucleolar localization is a result of the binding interactions of various domains of NSR1 within the nucleolus rather than the presence of a specific nucleolar targeting signal.
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