ArticleHistochemistry1993
Remodelling of cardiomyocyte cytoarchitecture visualized by three-dimensional (3D) confocal microscopy.
Article in Histochemistry, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
20 citing papers in PubMed.
- Generation of left ventricle-like cardiomyocytes with improved structural, functional, and metabolic maturity from human pluripotent stem cells.Cell reports methods · 2023Article
- Application of plasma polymerized pyrrole nanoparticles to prevent or reduce de-differentiation of adult rat ventricular cardiomyocytes.Journal of materials science. Materials in medicine · 2021Article
- Extracellular Matrix in Regulation of Contractile System in Cardiomyocytes.International journal of molecular sciences · 2019Review
- β-Adrenergic regulation of cardiac type 2A protein phosphatase through phosphorylation of regulatory subunit B56δ at S573.Journal of molecular and cellular cardiology · 2018Article
- Disassembly of myofibrils and potential imbalanced forces on Z-discs in cultured adult cardiomyocytes.Cytoskeleton (Hoboken, N.J.) · 2016Article
- Developmental regulation of MURF E3 ubiquitin ligases in skeletal muscle.Journal of muscle research and cell motility · 2012Article
- Recent advances in morphological cell image analysis.Computational and mathematical methods in medicine · 2012Review
- Developmental regulation of MURF ubiquitin ligases and autophagy proteins nbr1, p62/SQSTM1 and LC3 during cardiac myofibril assembly and turnover.Developmental biology · 2011Article
- Proteomics analysis of the cardiac myofilament subproteome reveals dynamic alterations in phosphatase subunit distribution.Molecular & cellular proteomics : MCP · 2010Article
- Distinct sarcomeric substrates are responsible for protein kinase D-mediated regulation of cardiac myofilament Ca2+ sensitivity and cross-bridge cycling.The Journal of biological chemistry · 2010Article
- Myofibrillogenesis in the developing zebrafish heart: A functional study of tnnt2.Developmental biology · 2009Article
- Adult rat cardiac myocytes in culture: 'Second-floor' cells and coculture experiments.Experimental and clinical cardiology · 2006Article
- Alterations at the intercalated disk associated with the absence of muscle LIM protein.The Journal of cell biology · 2001Article
- Cardiomyocyte cytoskeleton and myofibrillogenesis in healthy and diseased heart.Heart failure reviews · 2000Review
- Distinct families of Z-line targeting modules in the COOH-terminal region of nebulin.The Journal of cell biology · 2000Article
- Thin filament protein dynamics in fully differentiated adult cardiac myocytes: toward a model of sarcomere maintenance.The Journal of cell biology · 1999Article
- Different domains of the M-band protein myomesin are involved in myosin binding and M-band targeting.Molecular biology of the cell · 1999Article
- Point mutations in human beta cardiac myosin heavy chain have differential effects on sarcomeric structure and assembly: an ATP binding site change disrupts both thick and thin filaments, whereas hypertrophic cardiomyopathy mutations display normal assembly.The Journal of cell biology · 1997Article
- Triiodothyronine induces over-expression of alpha-smooth muscle actin, restricts myofibrillar expansion and is permissive for the action of basic fibroblast growth factor and insulin-like growth factor I in adult rat cardiomyocytes.The Journal of clinical investigation · 1996Article
- Dominant negative effect of cytoplasmic actin isoproteins on cardiomyocyte cytoarchitecture and function.The Journal of cell biology · 1995Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The break-down and reassembly of myofibrils in long-term cultures of adult rat cardiomyocytes was investigated by a novel combination of confocal laser scanning microscopy and three-dimensional image reconstruction, referred to as FTCS, to visualize the morphological changes these cells undergo in culture. FTCS is discussed as an alternative imaging mode to low-magnification scanning electron microscopy. The three-dimensional shape of the cells are correlated with the assembly state of myofibrils in different stages. Based on immunofluorescence and confocal laser scanning microscopy it was shown that myofibrils are degraded within a few days after plating and that newly assembled myofibrils are predominantly confined to the continuous area in the perinuclear region close to the membrane in contact with the substratum. The localization of myofibrils along the cell's vertical axis has been investigated both by optical sectioning using confocal light microscopy and by physical sectioning followed by transmission electron microscopy. Based on the distribution of myofibrillar proteins we propose a model of myofibrillar growth locating the putative assembly sites to a region concentric around the nuclei. We provide evidence that the cell shape is dominated by the myofibrillar apparatus.
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Registered trials
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