ArticleGenetics1993
Sequence requirements for myosin gene expression and regulation in Caenorhabditis elegans.
Article in Genetics, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 234 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
234 citing papers in PubMed, 436 citations in OpenAlex.
- Polyphyletic screen defines distinct classes of plant-derived natural products that oppose tauopathy.Life science alliance · 2026Article
- TDP-43 proteinopathies and neurodegeneration: insights from Caenorhabditis elegans models.The FEBS journal · 2026Review
- The Sole Essential Low Molecular Weight Tropomyosin Isoform of Caenorhabditis elegans Is Essential for Pharyngeal Muscle Function.Cytoskeleton (Hoboken, N.J.) · 2025Article
- Reduced Cas9 transgene silencing by incorporation of intron sequences.Nature communications · 2025Article
- Ribosomal protein mutation suppresses gonadal leader cell migration defects in mig-17/ADAMTS mutants in Caenorhabditis elegans.Scientific reports · 2025Article
- Parameters that influence bipartite reporter system expression in Caenorhabditis elegans.Genetics · 2025Article
- Introns increase gene expression in Caenorhabditis elegans by a mechanism that must be at least partly different than in plants.Scientific reports · 2025Article
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- Detecting gene expression in Caenorhabditis elegans.Genetics · 2025Review
- A versatile site-directed gene trap strategy to manipulate gene activity and control gene expression in Caenorhabditis elegans.PLoS genetics · 2025Article
- The sole essential low molecular weight tropomyosin isoform ofbioRxiv : the preprint server for biology · 2024Article
- RABR-1, an atypical Rab-related GTPase, cell-nonautonomously restricts somatosensory dendrite branching.Genetics · 2024Article
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- Silica Nanoparticles Disclose a Detailed Neurodegeneration Profile throughout the Life Span of a Model Organism.Journal of xenobiotics · 2024Article
- Overexpression of Lifeact in theFrontiers in cell and developmental biology · 2024Article
- Semantic representation of neural circuit knowledge in Caenorhabditis elegans.Brain informatics · 2023Article
- The α-arrestin SUP-13/ARRD-15 promotes isoform turnover of actin-interacting protein 1 inPNAS nexus · 2023Article
- An expanded GCaMP reporter toolkit for functional imaging in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2023Article
- An intestinal sphingolipid confers intergenerational neuroprotection.Nature cell biology · 2023Article
174 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Four Caenorhabditis elegans genes encode muscle-type specific myosin heavy chain isoforms: myo-1 and myo-2 are expressed in the pharyngeal muscles; unc-54 and myo-3 are expressed in body wall muscles. We have used transformation-rescue and lacZ fusion assays to determine sequence requirements for regulated myosin gene expression during development. Multiple tissue-specific activation elements are present for all four genes. For each of the four genes, sequences upstream of the coding region are tissue-specific promoters, as shown by their ability to drive expression of a reporter gene (lacZ) in the appropriate muscle type. Each gene contains at least one additional tissue-specific regulatory element, as defined by the ability to enhance expression of a heterologous promoter in the appropriate muscle type. In rescue experiments with unc-54, two further requirements apparently independent of tissue specificity were found: sequences within the 3' non-coding region are essential for activity while an intron near the 5' end augments expression levels. The general intron stimulation is apparently independent of intron sequence, indicating a mechanistic effect of splicing. To further characterize the myosin gene promoters and to examine the types of enhancer sequences in the genome, we have initiated a screen of C. elegans genomic DNA for fragments capable of enhancing the myo-2 promoter. The properties of enhancers recovered from this screen suggest that the promoter is limited to muscle cells in its ability to respond to enhancers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.