Evidence map›Paper›PMID 8242233›Full record

ArticleBritish journal of pharmacology1993

Single channel and whole-cell K-currents evoked by levcromakalim in smooth muscle cells from the rabbit portal vein.

D J Beech, H Zhang, K Nakao, T B Bolton

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in British journal of pharmacology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
3.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 92 citations in OpenAlex.

  1. Article
  2. ATP-sensitive KRespiratory physiology & neurobiology · 2020
    Article
  3. Article
  4. Metabolic hyperemia requires ATP-sensitive K+ channels and H2O2 but not adenosine in isolated mouse hearts.American journal of physiology. Heart and circulatory physiology · 2014
    Article
  5. Hypoxic pulmonary vasoconstriction.Physiological reviews · 2012
    Review
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  7. Review
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  18. Article
  19. Pharmacology of the potassium channel openers.Cardiovascular drugs and therapy · 1995
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

D J BeechDepartment of Pharmacology and Clinical Pharmacology, St. George's Hospital Medical School, London.
H Zhang
K Nakao
T B Bolton
St George's, University of London · GB

Funding

Wellcome Trust
6 · The paper itself

Abstract

1. Single channel and whole-cell current recordings were made from single smooth muscle cells isolated from the rabbit portal vein. 2. Application of 10 microM levcromakalim ((-)-Ckm) to single cells held with pipettes containing 1 mM GDP induced a K-current (IK(Ckm)) which occurred in addition to the current caused by GDP alone (IK(GDP)) and averaged 135 pA at -37 mV. We have investigated whether the same K channels underlie the GDP- and Ckm-induced K-currents. 3. If 1 mM GDP was in the pipette but Mg ions were omitted the effect of GDP was absent and IK(Ckm) averaged only 10 pA, suggesting that the action of (-)-Ckm was Mg-dependent. 4. Intracellular ATP was not observed to have much effect on IK(-Ckm). Loading of cells with 10 mM ATP from the recording pipette had no significant effect and flash photolysis of caged-ATP loaded into cells from the pipette, estimated to release about 1 mM free ATP, also had no effect on IK(-Ckm). 5. Bath-applied glibenclamide inhibited IK(-Ckm) with an IC50 of 200 nM, a value 8 times higher than that found for inhibition of IK(GDP). The delayed rectifier K-current (IK(DR)) was also inhibited by glibenclamide but at higher concentrations (IC50 100 microM). Bath-applied tetraethylammonium ions (TEA) inhibited IK(-Ckm) and IK(GDP) to the same extent (IC50 about 7 mM). 6. In inside-out patch recordings (- )-Ckm (10 microM) applied to the intracellular surface of the membrane potentiated the opening of K channels already stimulated by I mM GDP and all of the channel activity was abolished by 10 microM glibenclamide. The unitary conductance of the channels was 24lpS in a 60 mM: 130 mM K-gradient.7. We suggest that (-)-Ckm may hyperpolarize and relax smooth muscle cells by opening KNDP, a class of small conductance K channels that are related to the ATP-sensitive K channels seen in other tissues.

Indexed as

Adenosine TriphosphateAnimalsBenzopyransCromakalimGlyburideGuanosine DiphosphateIn Vitro TechniquesMagnesiumMuscle, Smooth, VascularPortal VeinPotassium ChannelsPyrrolesRabbitsTetraethylammonium CompoundsVasodilator AgentsAdenosine TriphosphateBenzopyransCromakalimGlyburideGuanosine DiphosphateMagnesiumPotassium ChannelsPyrrolesTetraethylammonium CompoundsVasodilator Agents

Identifiers

PMID8242233
PMCPMC2175920
OpenAlexW2030223575

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.